Anticorps Monoclonal anti-DICER1

DICER1 Monoclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1C8E2

N° de cat : 68375-1-PBS

Synonymes

DCR1, Dicer, dicer 1, ribonuclease type III, DICER1, Endoribonuclease Dicer, Helicase MOI, Helicase with RNase motif, HERNA, KIAA0928

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Galerie de données de validation

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  WB analysis of U2OS using 68375-1-Ig (same clone as 68375-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 68375-1-Ig (DICER1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68375-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 68375-1-Ig (same clone as 68375-1-PBS)

  IP result of anti-DICER1 (IP:68375-1-Ig, 4ug; Detection:68375-1-Ig 1:500) with Jurkat cells lysate 1560 ug. This data was developed using the same antibody clone with 68375-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68375-1-Ig (same clone as 68375-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DICER1 antibody (68375-1-Ig, Clone: 1C8E2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68375-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68375-1-PBS cible DICER1 dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DICER1 Protéine recombinante Ag30368
• Nom complet: dicer 1, ribonuclease type III
• Masse moléculaire calculée: 219 kDa
• Poids moléculaire observé: 240-250 kDa
• Numéro d’acquisition GenBank: NM_030621
• Symbole du gène: DICER1
• Identification du gène (NCBI): 23405
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DICER1 functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. DICER1 cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, and producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation.

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