Anticorps Polyclonal de lapin anti-DIDO1

DIDO1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10183-1-PBS

Synonymes

BYE1, C20orf158, DATF 1, DATF1, DATF-1

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Galerie de données de validation

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  WB analysis of Raji using 10183-1-AP (same clone as 10183-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 10183-1-AP (DIDO1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10183-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 10183-1-AP (same clone as 10183-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10183-1-AP (DIDO1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10183-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 10183-1-AP (same clone as 10183-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10183-1-AP (DIDO1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10183-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 10183-1-AP (same clone as 10183-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DIDO1 antibody (10183-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 10183-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 10183-1-AP (same clone as 10183-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using DIDO1 antibody 10183-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 10183-1-PBS in a different storage buffer formulation.

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Informations sur le produit

10183-1-PBS cible DIDO1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DIDO1 Protéine recombinante Ag0237
• Nom complet: death inducer-obliterator 1
• Masse moléculaire calculée: 244 kDa
• Poids moléculaire observé: 300-350 kDa
• Numéro d’acquisition GenBank: BC000770
• Symbole du gène: DIDO1
• Identification du gène (NCBI): 11083
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DIDO1, also termed DATF-1, C20orf158, DATF1, KIAA0333 and DIO-1, is a putative transcription factor, weakly pro-apoptotic when overexpressed. It is a tumor suppressor. In mice, DIDO1 is upregulated by apoptotic signals and encodes a cytoplasmic protein that translocates to the nucleus upon apoptotic signal activation. When overexpressed, the mouse protein induced apoptosis in cell lines growing in vitro. The human DIDO1 is similar to the mouse gene and therefore is thought to be involved in apoptosis. DIDO1 has multiple isoforms with molecular weights of 243 kd, 129 kd, 59 kd, and 61 kd. DIDO1 undergoes various modifications, including phosphorylation and ubiquitination, resulting in a mainstream molecular weight of 300 kd.

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