Anticorps Monoclonal anti-DIO2

• Phare
• Validé par KD/KO

DIO2 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

1F2A1

N° de cat : 66813-1-PBS

Synonymes

5DII, D2, DIOII, EC:1.21.99.4, ITDI2

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Galerie de données de validation

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  WB analysis of T-47D using 66813-1-Ig (same clone as 66813-1-PBS)

  T-47D cells were subjected to SDS PAGE followed by western blot with 66813-1-Ig (DIO2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66813-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 66813-1-Ig (same clone as 66813-1-PBS)

  WB result of DIO2 antibody (66813-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DIO2 transfected MCF-7 cells. This data was developed using the same antibody clone with 66813-1-PBS in a different storage buffer formulation.

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  WB analysis of LNCaP using 66813-1-Ig (same clone as 66813-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 66813-1-Ig (DIO2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66813-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 66813-1-Ig (same clone as 66813-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66813-1-Ig (DIO2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66813-1-PBS in a different storage buffer formulation.

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  IHC staining of human thyroid cancer using 66813-1-Ig (same clone as 66813-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 66813-1-Ig (DIO2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66813-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 66813-1-Ig (same clone as 66813-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66813-1-Ig (DIO2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66813-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 66813-1-Ig (same clone as 66813-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66813-1-Ig (DIO2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66813-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66813-1-PBS cible DIO2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DIO2 Protéine recombinante Ag24057
• Nom complet: deiodinase, iodothyronine, type II
• Masse moléculaire calculée: 273 aa, 31 kDa
• Poids moléculaire observé: 30-35 kDa
• Numéro d’acquisition GenBank: BC074882
• Symbole du gène: DIO2
• Identification du gène (NCBI): 1734
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Type II iodothyronine deiodinase(DIO2), belongs to the iodothyronine deiodinase family, with two isoform of 30 and 34 kDa. DIO2 can activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 3,3',5-triiodothyronine (T3). DIO2 is thought to be responsible for the 'local' production of T3, and thus important in influencing thyroid hormone action in these tissues.

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