Anticorps Polyclonal de lapin anti-DIS3

DIS3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 29664-1-PBS

Synonymes

DIS 3, Dis-3, dis3p, EC:3.1.13.-, EXOSC11

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Galerie de données de validation

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  WB analysis using 29664-1-AP (same clone as 29664-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29664-1-AP (DIS3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29664-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 29664-1-AP (same clone as 29664-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29664-1-AP (DIS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29664-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 29664-1-AP (same clone as 29664-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29664-1-AP (DIS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29664-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 29664-1-AP (same clone as 29664-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29664-1-AP (DIS3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29664-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 29664-1-AP (same clone as 29664-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 29664-1-AP (DIS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29664-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 29664-1-AP (same clone as 29664-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 29664-1-AP (DIS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29664-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 29664-1-AP (same clone as 29664-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29664-1-AP (DIS3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29664-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 29664-1-AP (same clone as 29664-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29664-1-AP (DIS3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29664-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 29664-1-AP (same clone as 29664-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 29664-1-AP (DIS3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29664-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 29664-1-AP (same clone as 29664-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DIS3 antibody (29664-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidine (red). This data was developed using the same antibody clone with 29664-1-PBS in a different storage buffer formulation.

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Informations sur le produit

29664-1-PBS cible DIS3 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DIS3 Protéine recombinante Ag31711
• Nom complet: DIS3 mitotic control homolog (S. cerevisiae)
• Masse moléculaire calculée: 109KD
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: NM_014953
• Symbole du gène: DIS3
• Identification du gène (NCBI): 22894
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DIS3, also named as KIAA1008 and RRP44, belongs to the ribonuclease II (RNB) family. It is a component of the exosome 3'->5' exoribonuclease complex. DIS3 is required for the 3'-processing of the 7S pre-RNA to the mature nuclear complex. It is also associated with the GTPase Ran. DIS3 has a 3'-5' exonuclease activity.

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