Anticorps Monoclonal anti-DIS3L2

DIS3L2 Monoclonal Antibody for WB, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat

Applications

WB, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2C9F2

N° de cat : 67623-1-PBS

Synonymes

DIS3 like exonuclease 2, DIS3-like exonuclease 2, EC:3.1.13.-, FAM6A, hDIS3L2

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Galerie de données de validation

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  WB analysis using 67623-1-Ig (same clone as 67623-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67623-1-Ig (DIS3L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67623-1-PBS in a different storage buffer formulation.

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  WB analysis using 67623-1-Ig (same clone as 67623-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67623-1-Ig (DIS3L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67623-1-PBS in a different storage buffer formulation.

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  FC experiment of Jurkat using 67623-1-Ig (same clone as 67623-1-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug DIS3L2 Monoclonal antibody (67623-1-Ig, Clone:2C9F2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67623-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67623-1-PBS cible DIS3L2 dans les applications de WB, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DIS3L2 Protéine recombinante Ag29992
• Nom complet: DIS3 mitotic control homolog (S. cerevisiae)-like 2
• Masse moléculaire calculée: 885 aa, 99 kDa
• Poids moléculaire observé: 65 kDa, 99 kda
• Numéro d’acquisition GenBank: BC030113
• Symbole du gène: DIS3L2
• Identification du gène (NCBI): 129563
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DIS3L2 encodes a protein of 885 amino acids with a predicted molecular weight of 99.3 kDa.

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