Anticorps Polyclonal de lapin anti-DKC1

• Phare
• Validé par KD/KO

DKC1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 25420-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HepG2 using 25420-1-AP (same clone as 25420-1-PBS)

  WB result of DKC1 antibody (25420-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-DKC1 transfected HepG2 cells. This data was developed using the same antibody clone with 25420-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 25420-1-AP (same clone as 25420-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 25420-1-AP (DKC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25420-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 25420-1-AP (same clone as 25420-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25420-1-AP (DKC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25420-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 25420-1-AP (same clone as 25420-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25420-1-AP (DKC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25420-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 25420-1-AP (same clone as 25420-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25420-1-AP (DKC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25420-1-PBS in a different storage buffer formulation.

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  WB analysis of COLO 320 using 25420-1-AP (same clone as 25420-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 25420-1-AP (DKC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25420-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 25420-1-AP (same clone as 25420-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25420-1-AP (DKC1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25420-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 25420-1-AP (same clone as 25420-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25420-1-AP (DKC1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25420-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 25420-1-AP (same clone as 25420-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 25420-1-AP (DKC1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25420-1-PBS in a different storage buffer formulation.

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Informations sur le produit

25420-1-PBS cible DKC1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DKC1 Protéine recombinante Ag7992
• Nom complet: dyskeratosis congenita 1, dyskerin
• Masse moléculaire calculée: 58 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: BC009928
• Symbole du gène: DKC1
• Identification du gène (NCBI): 1736
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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