Anticorps Recombinant de lapin anti-DKK1

DKK1 Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

250658B2

N° de cat : 86207-1-PBS

Synonymes

Dickkopf-1, Dickkopf-related protein 1, DKK 1, Dkk-1, hDkk 1

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Galerie de données de validation

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  WB analysis using 86207-1-RR (same clone as 86207-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86207-1-RR (DKK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86207-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP02491-1

  Sandwich ELISA standard curve of MP02491-1, Mouse DKK1 Recombinant Matched Antibody Pair - PBS only. 86207-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg4649. 86207-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

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  Sample test of MP02491-1

  Serum of eight mice was measured. The mouse DKK1 concentration of detected samples was determined to be 4.72 ng/mL with a range of 2.40 - 7.06 ng/mL.

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Informations sur le produit

86207-1-PBS cible DKK1 dans les applications de WB, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: DKK1 Protéine recombinante Eg4649
• Nom complet: dickkopf homolog 1 (Xenopus laevis)
• Masse moléculaire calculée: 29kDa
• Poids moléculaire observé: 30-35 kDa
• Numéro d’acquisition GenBank: NM_010051.3
• Symbole du gène: Dkk1
• Identification du gène (NCBI): 13380
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DKK1, also named a SK and Dickkopf-1, belongs to the dickkopf family. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. SDS-PAGE and Western blot analysis demonstrated that DKK1 is expressed as a 35-kDa doublet protein, which is larger than the deduced molecular mass of 26 kDa. Sometime DKK1 is expressed as a 42- to 50-kDa secreted protein, with little change observed after glycanase treatment.

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