Anticorps Monoclonal anti-DKK3

DKK3 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(3)

CloneNo.

4E6H6

N° de cat : 66758-1-Ig

Synonymes

Dickkopf 3, Dickkopf related protein 3, Dkk 3, DKK3, hDkk 3, REIC, RIG

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Galerie de données de validation

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  WB analysis using 66758-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66758-1-Ig (DKK3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 66758-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66758-1-Ig (DKK3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 66758-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66758-1-Ig (DKK3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 66758-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66758-1-Ig (DKK3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 66758-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66758-1-Ig (DKK3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 66758-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using DKK3 antibody (66758-1-Ig, Clone: 4E6H6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules MCF-7, cellules NCCIT
• Résultats positifs en IHC: tissu cardiaque humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:150-1:600
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

66758-1-Ig cible DKK3 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DKK3 Protéine recombinante Ag23615
• Nom complet: dickkopf homolog 3 (Xenopus laevis)
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 40 kDa, 50-55 kDa
• Numéro d’acquisition GenBank: BC007660
• Symbole du gène: DKK3
• Identification du gène (NCBI): 27122
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DKK3, also named as REIC, belongs to the dickkopf family. It antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer's disea. The adenovirus carrying REIC/Dkk-3 (Ad-REIC) exhibits a potent tumor-specific cell-killing function for various human cancers. It has also become evident that some human cancers are resistant to Ad-REIC-induced apoptosis. The DKK3 is a Glycosylation protein with MW about 55 kDa or 38-43 kDa.

Publications

Species	Application	Title
pig	WB	Reprod Biol Endocrinol; Extracellular vesicles derived from endometrial epithelial cells deliver exogenous miR-92b-3p to affect the function of embryonic trophoblast cells via targeting TSC1 and DKK3; Authors - Renwu Hua
human	WB,IF	Nat Commun; A single cell atlas of frozen shoulder capsule identifies features associated with inflammatory fibrosis resolution; Authors - Michael T H Ng
mouse	WB,IF	Brain Behav Immun; Mechanistic insights into the neuroprotective effects of low-intensity transcranial ultrasound stimulation in post-cardiac arrest brain injury: Modulation of the Piezo1-Dkk3/PI3K-Akt pathway; Authors - Shuang Xu

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