Anticorps Monoclonal anti-DKK3

DKK3 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4E6H6

N° de cat : 66758-1-PBS

Synonymes

Dickkopf 3, Dickkopf related protein 3, Dkk 3, DKK3, hDkk 3, REIC, RIG

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Galerie de données de validation

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  WB analysis using 66758-1-Ig (same clone as 66758-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66758-1-Ig (DKK3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66758-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 66758-1-Ig (same clone as 66758-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66758-1-Ig (DKK3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66758-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66758-1-Ig (same clone as 66758-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66758-1-Ig (DKK3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66758-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 66758-1-Ig (same clone as 66758-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66758-1-Ig (DKK3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66758-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66758-1-Ig (same clone as 66758-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66758-1-Ig (DKK3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66758-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 66758-1-Ig (same clone as 66758-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using DKK3 antibody (66758-1-Ig, Clone: 4E6H6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66758-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66758-1-PBS cible DKK3 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DKK3 Protéine recombinante Ag23615
• Nom complet: dickkopf homolog 3 (Xenopus laevis)
• Masse moléculaire calculée: 38 kDa
• Poids moléculaire observé: 40 kDa, 50-55 kDa
• Numéro d’acquisition GenBank: BC007660
• Symbole du gène: DKK3
• Identification du gène (NCBI): 27122
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DKK3, also named as REIC, belongs to the dickkopf family. It antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer's disea. The adenovirus carrying REIC/Dkk-3 (Ad-REIC) exhibits a potent tumor-specific cell-killing function for various human cancers. It has also become evident that some human cancers are resistant to Ad-REIC-induced apoptosis. The DKK3 is a Glycosylation protein with MW about 55 kDa or 38-43 kDa.

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