Anticorps Monoclonal anti-DLAT

• Phare
• Validé par KD/KO

DLAT Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1F2B9

N° de cat : 68303-1-PBS

Synonymes

1F2B9, Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial, DLTA, EC:2.3.1.12, ODP2

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Galerie de données de validation

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  WB analysis using 68303-1-Ig (same clone as 68303-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68303-1-Ig (DLAT antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68303-1-PBS in a different storage buffer formulation.

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  WB analysis of HAP1 using 68303-1-Ig (same clone as 68303-1-PBS)

  WB result of DLAT antibody (68303-1-Ig; 1:20000; room temperature for 1.5 hours) with wild-type and sg-DLAT transfected HAP1 cells. This data was developed using the same antibody clone with 68303-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 68303-1-Ig (same clone as 68303-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68303-1-Ig (DLAT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68303-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 68303-1-Ig (same clone as 68303-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68303-1-Ig (DLAT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68303-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 68303-1-Ig (same clone as 68303-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68303-1-Ig (DLAT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68303-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 68303-1-Ig (same clone as 68303-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68303-1-Ig (DLAT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68303-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68303-1-Ig (same clone as 68303-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DLAT antibody (68303-1-Ig, Clone: 1F2B9 ) at dilution of 1:500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 68303-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68303-1-Ig (same clone as 68303-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DLAT antibody (68303-1-Ig, Clone: 1F2B9 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68303-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68303-1-PBS cible DLAT dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DLAT Protéine recombinante Ag4226
• Nom complet: dihydrolipoamide S-acetyltransferase
• Masse moléculaire calculée: 647 aa, 70 kDa
• Poids moléculaire observé: 69-70 kDa
• Numéro d’acquisition GenBank: BC039084
• Symbole du gène: DLAT
• Identification du gène (NCBI): 1737
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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