Anticorps Monoclonal anti-DLD

• Phare
• Validé par KD/KO

DLD Monoclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2E7G5

N° de cat : 67702-1-PBS

Synonymes

2E7G5, DLDH, EC:1.8.1.4, GCSL, LAD

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Galerie de données de validation

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  WB analysis using 67702-1-Ig (same clone as 67702-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67702-1-Ig (DLD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67702-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67702-1-Ig (same clone as 67702-1-PBS)

  WB result of DLD antibody (67702-1-Ig; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DLD transfected HeLa cells. This data was developed using the same antibody clone with 67702-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 67702-1-Ig (same clone as 67702-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67702-1-Ig (DLD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67702-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67702-1-Ig (same clone as 67702-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67702-1-Ig (DLD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67702-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67702-1-Ig (same clone as 67702-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67702-1-Ig (DLD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67702-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 67702-1-Ig (same clone as 67702-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67702-1-Ig (DLD antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67702-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67702-1-PBS cible DLD dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DLD Protéine recombinante Ag9680
• Nom complet: dihydrolipoamide dehydrogenase
• Masse moléculaire calculée: 509 aa, 54 kDa
• Poids moléculaire observé: 54 kDa
• Numéro d’acquisition GenBank: BC018696
• Symbole du gène: DLD
• Identification du gène (NCBI): 1738
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DLD(Dihydrolipoyl dehydrogenase, mitochondrial) is also named as GCSL, LAD, PHE3 and belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family. It catalyzes the oxidation of dihydrolipoamide, hE3 uses two molecules : non-covalently bound FAD and a transiently bound substrate, NAD+. DLD is involved in the hyperactivation of spermatazoa during capacitation and in the spermatazoal acrosome reaction.

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