- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-HURP
HURP Polyclonal Antibody for WB, IF/ICC, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain et plus (1)
Applications
WB, IHC, IF/ICC, ELISA
Conjugaison
Non conjugué
N° de cat : 12038-1-AP
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | cellules HeLa, cellules A431, cellules HEK-293, cellules PC-3, cellules U2OS |
| Résultats positifs en IF/ICC | cellules HEK-293 |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| KD/KO | See 4 publications below |
| WB | See 8 publications below |
| IHC | See 4 publications below |
| IF | See 1 publications below |
Informations sur le produit
12038-1-AP cible HURP dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain
| Réactivité | Humain |
| Réactivité citée | Humain, porc |
| Hôte / Isotype | Lapin / IgG |
| Clonalité | Polyclonal |
| Type | Anticorps |
| Immunogène | HURP Protéine recombinante Ag2667 |
| Nom complet | discs, large (Drosophila) homolog-associated protein 5 |
| Masse moléculaire calculée | 846 aa, 95 kDa |
| Poids moléculaire observé | 120 kDa |
| Numéro d’acquisition GenBank | BC010658 |
| Symbole du gène | HURP |
| Identification du gène (NCBI) | 9787 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par affinité contre l'antigène |
| Tampon de stockage | PBS with 0.02% sodium azide and 50% glycerol |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
DLGAP5, also named as DAP 5 or DLG7, is a kinetochore protein that stabilizes microtubules in vicinity of chromosomes. DLG7 controls spindle dynamics, promotes interkinetochore tension and efficient kinetochore capture. DGL7 is a part of Ran-dependent complex. Expression of DGL7 is found in cancer and stem cells. DLGAP5 contributes to hepatocellular carcinoma tumorigenesis by promoting cell proliferation (PMID: 24324629).
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for HURP antibody 12038-1-AP | Download protocol |
| IF protocol for HURP antibody 12038-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun HURP facilitates spindle assembly by stabilizing microtubules and working synergistically with TPX2 | ||
J Transl Med Integrative multi-omics analysis unveils stemness-associated molecular subtypes in prostate cancer and pan-cancer: prognostic and therapeutic significance | ||
J Transl Med DLGAP5 promotes lung adenocarcinoma growth via upregulating PLK1 and serves as a therapeutic target
| ||
Cells Mitotic Acetylation of Microtubules Promotes Centrosomal PLK1 Recruitment and Is Required to Maintain Bipolar Spindle Homeostasis. | ||
Life Sci Pan-cancer analysis and experiments with cell lines reveal that the slightly elevated expression of DLGAP5 is involved in clear cell renal cell carcinoma progression.
| ||
Front Endocrinol (Lausanne) Whole Genome Transcriptomic Analysis of Ovary Granulosa Cells Revealed an Anti-Apoptosis Regulatory Gene DLGAP5 in Polycystic Ovary Syndrome. |

