Anticorps Polyclonal de lapin anti-HURP

• Phare
• Validé par KD/KO

HURP Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(11)

N° de cat : 12038-1-AP

Synonymes

DAP-5, Discs large homolog 7, Disks large-associated protein 5, Disks large-associated protein DLG7, DLG7

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Galerie de données de validation

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  WB analysis using 12038-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12038-1-AP (HURP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 12038-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 12038-1-AP (HURP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A431, cellules HEK-293, cellules PC-3, cellules U2OS
• Résultats positifs en IF/ICC: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 8 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

12038-1-AP cible HURP dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: HURP Protéine recombinante Ag2667
• Nom complet: discs, large (Drosophila) homolog-associated protein 5
• Masse moléculaire calculée: 846 aa, 95 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: BC010658
• Symbole du gène: HURP
• Identification du gène (NCBI): 9787
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DLGAP5, also named as DAP 5 or DLG7, is a kinetochore protein that stabilizes microtubules in vicinity of chromosomes. DLG7 controls spindle dynamics, promotes interkinetochore tension and efficient kinetochore capture. DGL7 is a part of Ran-dependent complex. Expression of DGL7 is found in cancer and stem cells. DLGAP5 contributes to hepatocellular carcinoma tumorigenesis by promoting cell proliferation (PMID: 24324629).

Publications

Species	Application	Title
	WB	Nat Commun; HURP facilitates spindle assembly by stabilizing microtubules and working synergistically with TPX2; Authors - Venecia Alexandria Valdez
human	IHC	J Transl Med; Integrative multi-omics analysis unveils stemness-associated molecular subtypes in prostate cancer and pan-cancer: prognostic and therapeutic significance; Authors - Kun Zheng
human	WB,IHC	J Transl Med; DLGAP5 promotes lung adenocarcinoma growth via upregulating PLK1 and serves as a therapeutic target; Authors - Maojian Chen ; KD Validated
pig	IF	Cells; Mitotic Acetylation of Microtubules Promotes Centrosomal PLK1 Recruitment and Is Required to Maintain Bipolar Spindle Homeostasis.; Authors - Sylvia Fenosoa Rasamizafy
human	WB,IHC	Life Sci; Pan-cancer analysis and experiments with cell lines reveal that the slightly elevated expression of DLGAP5 is involved in clear cell renal cell carcinoma progression.; Authors - Yun Feng ; KD Validated
human	WB	Front Endocrinol (Lausanne); Whole Genome Transcriptomic Analysis of Ovary Granulosa Cells Revealed an Anti-Apoptosis Regulatory Gene DLGAP5 in Polycystic Ovary Syndrome.; Authors - Yan Deng

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