Anticorps Polyclonal de lapin anti-DLL1

DLL1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 28544-1-AP

Synonymes

H-Delta-1, Drosophila Delta homolog 1, Delta-like protein 1, Delta1, Delta like protein 1

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Galerie de données de validation

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  WB analysis using 28544-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28544-1-AP (DLL1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 28544-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28544-1-AP (DLL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu cérébral de rat, cellules HeLa, cellules HUVEC, tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below

Informations sur le produit

28544-1-AP cible DLL1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DLL1 Protéine recombinante Ag29534
• Nom complet: delta-like 1 (Drosophila)
• Masse moléculaire calculée: 78 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: NM_005618
• Symbole du gène: DLL1
• Identification du gène (NCBI): 28514
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The Notch pathway functions during diverse developmental and physiological processes. Notch receptor and its ligands, Delta and Jagged, are transmembrane proteins with large extracellular domains that consist primarily of epidermal growth factor (EGF)-like repeats (PMID: 16921404). Delta-like protein 1 (DLL1, also named as Delta1) is a ligand of NOTCH1, NOTCH2 and NOTCH3 receptors that binds the extracellular domain of Notch receptor in a cis and trans fashion manner (PMID: 11006133). DLL1 play a role in development of the central nervous system, somites and lymphocytes (PMID: 31353024). It is overexpressed in various cancers and is involved in the regulation of cancer progression (PMID: 32440154). DLL1 undergoes proteolytic processing in its extracellular domain by ADAM proteases and gamma-secretase (PMID: 17107962; 12794186).

Publications

Species	Application	Title
mouse	WB	Phytomedicine; Celastrol ameliorates fibrosis in Western diet/tetrachloromethane-induced nonalcoholic steatohepatitis by suppressing Notch/osteopontin signaling; Authors - Dingwu Li
human	WB	ACS Omega; Polyethylenimine Triggers Dll4 Degradation to Regulate Angiogenesis In Vitro; Authors - Binghua Cheng
human	WB	Drug Deliv Transl Res; LINC00460 promotes neuroblastoma tumorigenesis and cisplatin resistance by targeting miR-149-5p/DLL1 axis and activating Notch pathway in vitro and in vivo; Authors - Yali Xu
mouse	WB	Int Immunopharmacol; Artemether ameliorates DSS-induced ulcerative colitis by modulating macrophage polarization; Authors - Xinxin Ren

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