Anticorps Polyclonal de lapin anti-DLL4

• Phare
• Validé par KD/KO

DLL4 Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 21584-1-PBS

Synonymes

Delta4, hdelta2, UNQ1895/PRO4341

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Galerie de données de validation

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  WB analysis using 21584-1-AP (same clone as 21584-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21584-1-AP (DLL4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21584-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 21584-1-AP (same clone as 21584-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21584-1-AP (DLL4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21584-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse lung using 21584-1-AP (same clone as 21584-1-PBS)

  IP result of anti-DLL4 (IP:21584-1-AP, 4ug; Detection:21584-1-AP 1:300) with mouse lung tissue lysate 4000 ug. This data was developed using the same antibody clone with 21584-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 21584-1-AP (same clone as 21584-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 21584-1-AP (DLL4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21584-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 21584-1-AP (same clone as 21584-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 21584-1-AP (DLL4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21584-1-PBS in a different storage buffer formulation.

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  IF Staining of human stomach cancer using 21584-1-AP (same clone as 21584-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human stomach cancer tissue using 21584-1-AP (DLL4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 21584-1-PBS in a different storage buffer formulation.

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Informations sur le produit

21584-1-PBS cible DLL4 dans les applications de WB, IHC, IF-P, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DLL4 Protéine recombinante Ag16140
• Nom complet: delta-like 4 (Drosophila)
• Masse moléculaire calculée: 685 aa, 75 kDa
• Poids moléculaire observé: 70-100 kDa
• Numéro d’acquisition GenBank: BC106950
• Symbole du gène: DLL4
• Identification du gène (NCBI): 54567
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DLL4, also named as Delta4, plays a role in the Notch signaling pathway. It activates Notch-1 and Notch-4. Notch signaling is activated upon engagement of the Notch receptor with its ligands, the DSL (Delta, Serrate, Lag2) proteins of single-pass type I membrane proteins. DLL4 is highly expressed in the vascular endothelium and is involved in angiogenesis.

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