Anticorps Polyclonal de lapin anti-DMAP1

• Phare
• Validé par KD/KO

DMAP1 Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, RIP, IP, ChIP, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 10411-1-AP

Synonymes

DMAP1, DNMAP1, DNMT1 associated protein 1, DNMTAP1, EAF2, KIAA1425, SWC4

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Galerie de données de validation

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  WB analysis using 10411-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10411-1-AP (DMAP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 10411-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10411-1-AP (DMAP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 10411-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10411-1-AP (DMAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10411-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10411-1-AP (DMAP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 10411-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10411-1-AP (DMAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10411-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10411-1-AP (DMAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of COLO 320 using 10411-1-AP

  IP Result of anti-DMAP1 (IP:10411-1-AP, 3ug; Detection:10411-1-AP 1:300) with COLO 320 cells lysate 2500ug.

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules HeLa, cellules NIH/3T3
• Résultats positifs en IP: cellules COLO 320

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• ChIP: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

10411-1-AP cible DMAP1 dans les applications de WB, RIP, IP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DMAP1 Protéine recombinante Ag0665
• Nom complet: DNA methyltransferase 1 associated protein 1
• Masse moléculaire calculée: 53 kDa
• Poids moléculaire observé: 53-60 kDa
• Numéro d’acquisition GenBank: BC002855
• Symbole du gène: DMAP1
• Identification du gène (NCBI): 55929
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DMAP1 (DNA methyltransferase 1-associated protein 1) a component of the NuA4 histone acetyltransferase complex, was originally identified as an interacting molecule with DNA methyltransferase 1 (DNMT1) which co-localizes with PCNA at DNA replication foci. DMAP1 is required for DNA repair and acts against genomic instability. DMAP1 is widely implicated in transcription repression and activation via its interaction with multiple targets including histone deacetylase2 (HDAC2). The antibody reacts specifically with DMAP1 protein.

Publications

Species	Application	Title
human	WB	Cell; An RNAi screen of chromatin proteins identifies Tip60-p400 as a regulator of embryonic stem cell identity.; Authors - Thomas G Fazzio ; KD Validated
mouse		Nat Struct Mol Biol; R loops regulate promoter-proximal chromatin architecture and cellular differentiation.; Authors - Poshen B Chen
human	WB	EMBO Mol Med; Phosphorylation of TFCP2L1 by CDK1 is required for stem cell pluripotency and bladder carcinogenesis.; Authors - Jinbeom Heo
mouse	WB	Elife; Hdac6 regulates Tip60-p400 function in stem cells.; Authors - Chen Poshen B PB
mouse	WB	Cell Rep; Sirt1 Regulates DNA Methylation and Differentiation Potential of Embryonic Stem Cells by Antagonizing Dnmt3l.; Authors - Jinbeom Heo
mouse	ChIP,RIP	Psychiatry Investig; m6A Methyltransferase METTL3 Reduces Hippocampal Neuron Apoptosis in a Mouse Model of Autism Through the MALAT1/SFRP2/Wnt/β-catenin Axis; Authors - Yue Ming

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