Anticorps Monoclonal anti-DMC1

DMC1 Monoclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2D5C9

N° de cat : 67176-1-PBS

Synonymes

LIM15, HsLim15, DMC1H, 2D5C9

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Galerie de données de validation

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  WB analysis of HeLa using 67176-1-Ig (same clone as 67176-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67176-1-Ig (DMC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67176-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 67176-1-Ig (same clone as 67176-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67176-1-Ig (DMC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67176-1-PBS in a different storage buffer formulation.

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  WB analysis using 67176-1-Ig (same clone as 67176-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67176-1-Ig (DMC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67176-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse testis using 67176-1-Ig (same clone as 67176-1-PBS)

  IP result of anti-DMC1 (IP:67176-1-Ig, 4ug; Detection:67176-1-Ig 1:1000) with mouse testis tissue lysate 2040 ug. This data was developed using the same antibody clone with 67176-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 67176-1-Ig (same clone as 67176-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67176-1-Ig (DMC1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67176-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 67176-1-Ig (same clone as 67176-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67176-1-Ig (DMC1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67176-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 67176-1-Ig (same clone as 67176-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using DMC1 antibody (67176-1-Ig, Clone: 2D5C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). DAPI (blue). This data was developed using the same antibody clone with 67176-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse testis using 67176-1-Ig (same clone as 67176-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using DMC1 antibody (67176-1-Ig, Clone: 2D5C9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). DAPI (blue). This data was developed using the same antibody clone with 67176-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67176-1-PBS cible DMC1 dans les applications de WB, IHC, IF-P, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DMC1 Protéine recombinante Ag6951
• Nom complet: DMC1 dosage suppressor of mck1 homolog, meiosis-specific homologous recombination (yeast)
• Masse moléculaire calculée: 165 aa, 18 kDa, 38 kDa
• Poids moléculaire observé: 37 kDa
• Numéro d’acquisition GenBank: BC035658
• Symbole du gène: DMC1
• Identification du gène (NCBI): 11144
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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