Anticorps Recombinant de lapin anti-DMT1

DMT1 Recombinant Antibody for Indirect ELISA, IP, WB

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

Indirect ELISA, IP, WB

Conjugaison

Non conjugué

CloneNo.

1I13

N° de cat : 81609-1-PBS

Synonymes

SLC11A2, OK/SW-cl.20, Natural resistance-associated macrophage protein 2, DMT-1, DCT1

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Galerie de données de validation

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  WB analysis using 81609-1-RR (same clone as 81609-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81609-1-RR (DMT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81609-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 81609-1-RR (same clone as 81609-1-PBS)

  IP result of anti-DMT1 (IP:81609-1-RR, 4ug; Detection:81609-1-RR 1:2000) with HEK-293 cells lysate 1480 ug. This data was developed using the same antibody clone with 81609-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 81609-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81609-1-RR against Human DMT1 were performed. The affinity constant is 1.93 nM.

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Informations sur le produit

81609-1-PBS cible DMT1 dans les applications de Indirect ELISA, IP, WB et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: DMT1 Protéine recombinante Ag14314
• Nom complet: solute carrier family 11 (proton-coupled divalent metal ion transporters), member 2
• Masse moléculaire calculée: 568 aa, 62 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC002592
• Symbole du gène: DMT1
• Identification du gène (NCBI): 4891
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SLC11A2 (also known as DMT1, Nramp2, and DCT1) is a member of the divalent cation transporters that plays a central role in iron homeostasis. SLC11A2 is widely expressed in many tissues including brain, kidney, testis, duodenum and placenta. As a membrane protein, SLC11A2 is localized on the apical membrane of enterocytes as well as in transferrin-cycle endosomes. Four isoforms of SLC11A2 exist due to the alternative splicing. They differ at the NH2 and COOH termini but share a common central domain. A variety of molecular weights of SLC11A2 in western blot analysis, ranging from 50 to 100 kDa, has been reported in different cells and species. The differences in molecular weights may be attributed to the level of glycosylation, proteolysis, or the membrane protein itself. This antibody detected various forms of SLC11A2 of 45-100 kDa.

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