Anticorps Monoclonal anti-DMXL2

DMXL2 Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1F9A8

N° de cat : 66891-2-Ig

Synonymes

Rabconnectin 3, KIAA0856, DmX-like protein 2, DmX like protein 2, Dmx like 2

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Galerie de données de validation

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  WB analysis of mouse cerebellum using 66891-2-Ig

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 66891-2-Ig (DMXL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 66891-2-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66891-2-Ig (DMXL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 66891-2-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66891-2-Ig (DMXL2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of Jurkat using 66891-2-Ig

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using DMXL2 antibody (66891-2-Ig, Clone: 1F9A8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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Applications testées

• Résultats positifs en WB: tissu de cervelet de souris, cellules Jurkat, tissu cérébral de rat
• Résultats positifs en IF/ICC: cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66891-2-Ig cible DMXL2 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DMXL2 Protéine recombinante Ag19861
• Nom complet: Dmx-like 2
• Masse moléculaire calculée: 3036 aa, 340 kDa
• Poids moléculaire observé: 340 kDa
• Numéro d’acquisition GenBank: BC140781
• Symbole du gène: DMXL2
• Identification du gène (NCBI): 23312
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DMXL2, also named as rabconnectin-3, is a functional regulator of mammalian notch signaling with WD domains. Rabconnectin-3 is abundantly expressed in the brain where it is enriched in the synaptic vesicle fraction. Immunofluorescence and immunoelectron microscopy revealed that rabconnectin-3 was concentrated on synaptic vesicles at synapses. It may serve as a scaffold molecule for both Rab3 GEP and GAP on synaptic vesicles.

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