Anticorps Recombinant de lapin anti-DNA ligase III/LIG3

DNA ligase III/LIG3 Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242870C6

N° de cat : 85409-4-PBS

Synonymes

LIG3, 242870C6, DNA ligase 3, DNA ligase III, EC:6.5.1.1

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Galerie de données de validation

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  WB analysis using 85409-4-RR (same clone as 85409-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85409-4-RR (LIG3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85409-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85409-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85409-4-RR against Human DNA ligase III/LIG3 were performed. The affinity constant is 3.04 nM.

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Informations sur le produit

85409-4-PBS cible DNA ligase III/LIG3 dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: DNA ligase III/LIG3 Protéine recombinante Ag24917
• Nom complet: ligase III, DNA, ATP-dependent
• Masse moléculaire calculée: 100-110 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC068005
• Symbole du gène: LIG3
• Identification du gène (NCBI): 3980
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DNA ligase III (DNA ligase 3) is an enzyme that in humans is encoded by the LIG3 gene. The human LIG3 gene encodes ATP-dependent DNA ligases that seal interruptions in the phosphodiester backbone of duplex DNA. There are three families of ATP-dependent DNA ligases in eukaryotes. These enzymes utilize the same three step reaction mechanism; 1 formation of a covalent enzyme-adenylate intermediate; 2 transfer of the adenylate group to the 5' phosphate terminus of a DNA nick; 3 phosphodiester bond formation. Unlike LIG1 and LIG4 family members that are found in almost all eukaryotes, LIG3 family members are less widely distributed. The LIG3 gene encodes several distinct DNA ligase species by alternative translation initiation and alternative splicing mechanisms.

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