Anticorps Polyclonal de lapin anti-DNA2

• Phare
• Validé par KD/KO

DNA2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 18727-1-PBS

Synonymes

DNA2L, KIAA0083

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Galerie de données de validation

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  WB analysis of mouse liver using 18727-1-AP (same clone as 18727-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18727-1-AP (DNA2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18727-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse thymus using 18727-1-AP (same clone as 18727-1-PBS)

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 18727-1-AP (DNA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18727-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 18727-1-AP (same clone as 18727-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18727-1-AP (DNA2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18727-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse liver using 18727-1-AP (same clone as 18727-1-PBS)

  IP result of anti-DNA2 (IP:18727-1-AP, 4ug; Detection:18727-1-AP 1:300) with mouse liver tissue lysate 4800 ug. This data was developed using the same antibody clone with 18727-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 18727-1-AP (same clone as 18727-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18727-1-AP (DNA2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18727-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 18727-1-AP (same clone as 18727-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18727-1-AP (DNA2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18727-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 18727-1-AP (same clone as 18727-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 18727-1-AP (DNA2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18727-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 18727-1-AP (same clone as 18727-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DNA2 antibody (18727-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 18727-1-PBS in a different storage buffer formulation.

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Informations sur le produit

18727-1-PBS cible DNA2 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: DNA replication helicase 2 homolog (yeast)
• Masse moléculaire calculée: 120 kDa
• Poids moléculaire observé: 120-130 kDa
• Numéro d’acquisition GenBank: NM_001080449
• Symbole du gène: DNA2
• Identification du gène (NCBI): 1763
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DNA2(DNA replication ATP-dependent helicase/nuclease DNA2) is also named as DNA2L, KIAA0083 and belongs to the DNA2/NAM7 helicase family. This protein includes 2 domains: DNA replication nuclease and DNA replication ATP-dependent helicase. It is a conserved helicase/nuclease involved in the maintenance of mitochondrial and nuclear DNA stability and it can interact with DNA polymerase gamma and stimulating its activity. It can also process intermediate 5' flap structures occuring in DNA replication long-patch base excision repair in mitochondria. It has 4 isoforms produced by alternative splicing. This antibody is specific to DNA2.

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