Anticorps Polyclonal de lapin anti-DNAH5

DNAH5 Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 31079-1-PBS

Synonymes

KIAA1603, HL1, Dynein heavy chain 5, axonemal, DNAHC5, Ciliary dynein heavy chain 5

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Galerie de données de validation

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  IHC staining of human lung using 31079-1-AP (same clone as 31079-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 31079-1-AP (DNAH5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31079-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 31079-1-AP (same clone as 31079-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 31079-1-AP (DNAH5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31079-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 31079-1-AP (same clone as 31079-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DNAH5 antibody (31079-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 31079-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 31079-1-AP (same clone as 31079-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DNAH5 antibody (31079-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 31079-1-PBS in a different storage buffer formulation.

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Informations sur le produit

31079-1-PBS cible DNAH5 dans les applications de IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DNAH5 Protéine recombinante Ag34647
• Nom complet: dynein, axonemal, heavy chain 5
• Masse moléculaire calculée: 529 kDa
• Numéro d’acquisition GenBank: NM_001369
• Symbole du gène: DNAH5
• Identification du gène (NCBI): 1767
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Dynein axonemal heavy chain 5 (DNAH5) is an axonemal heavy chain dynein, part of a microtubule-associated motor protein complex. DNAH5 is a large gene comprising 79 exons and one alternative first exon and encodes a heavy chain of the ODA. We have recently shown that recessive DNAH5 mutations are responsible for PCD characterized by ODA defects. DNAH5 mutations cause PCD and randomization of left-right asymmetry (PMID: 15750039).

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