Anticorps Polyclonal de lapin anti-DNAH6

DNAH6 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 30073-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 30073-1-AP (same clone as 30073-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 30073-1-AP (DNAH6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30073-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 30073-1-AP (same clone as 30073-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30073-1-AP (DNAH6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30073-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 30073-1-AP (same clone as 30073-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30073-1-AP (DNAH6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30073-1-PBS in a different storage buffer formulation.

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  IF Staining of hTERT-RPE1 using 30073-1-AP (same clone as 30073-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using DNAH6 antibody (30073-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red). This data was developed using the same antibody clone with 30073-1-PBS in a different storage buffer formulation.

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  IF Staining of hTERT-RPE1 using 30073-1-AP (same clone as 30073-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using DNAH6 antibody (30073-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite® Plus 488 ARL13B antibody (CL488-17711, green). This data was developed using the same antibody clone with 30073-1-PBS in a different storage buffer formulation.

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Informations sur le produit

30073-1-PBS cible DNAH6 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DNAH6 Protéine recombinante Ag32364
• Nom complet: dynein, axonemal, heavy chain 6
• Masse moléculaire calculée: 475 kDa
• Poids moléculaire observé: 475 kDa
• Numéro d’acquisition GenBank: BC104884
• Symbole du gène: DNAH6
• Identification du gène (NCBI): 1768
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DNAH6(Dynein heavy chain 6, axonemal) is also named as DNAHC6, DNHL1, HL2, KIAA1697 and belongs to the dynein heavy chain family.Dyneins are microtubule-associated motor protein complexes composed of several heavy, light, and intermediate chains(PMID:8812413). It is a force generating protein of respiratory cilia and produces force towards the minus ends of microtubules.This antibody is specific to DNAH6.

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