Anticorps Polyclonal de lapin anti-DNAI2

DNAI2 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications(14)

N° de cat : 17533-1-AP

Synonymes

CILD9, Dynein axonemal intermediate chain 2

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Galerie de données de validation

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  WB analysis of mouse ovary using 17533-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 17533-1-AP (DNAI2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 17533-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 17533-1-AP (DNAI2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17533-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17533-1-AP (DNAI2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 17533-1-AP

  IP result of anti-DNAI2 (IP:17533-1-AP, 4ug; Detection:17533-1-AP 1:400) with mouse lung tissue lysate 4000ug.

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  IHC staining of human heart using 17533-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17533-1-AP (DNAI2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse ovary using 17533-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 17533-1-AP (DNAI2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse ovary using 17533-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 17533-1-AP (DNAI2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse testis using 17533-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using DNAI2 antibody (17533-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu ovarien de souris, cellules HeLa, tissu pulmonaire de souris
• Résultats positifs en IP: tissu pulmonaire de souris
• Résultats positifs en IHC: tissu ovarien de souris, tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu testiculaire de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 10 publications below
• IF: See 10 publications below
• IP: See 2 publications below

Informations sur le produit

17533-1-AP cible DNAI2 dans les applications de WB, IHC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DNAI2 Protéine recombinante Ag11585
• Nom complet: dynein, axonemal, intermediate chain 2
• Masse moléculaire calculée: 593 aa, 67 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC039582
• Symbole du gène: DNAI2
• Identification du gène (NCBI): 64446
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB,IP,IF	Elife; ZMYND10 functions in a chaperone relay during axonemal dynein assembly.; Authors - Girish R Mali
mouse	IF	J Mol Cell Biol; Production of transgenic mice by random recombination of targeted genes in female germline stem cells.; Authors - Zhang Yong Y
rat	WB,IF	Front Cell Dev Biol; PIH1D3-knockout rats exhibit full ciliopathy features and dysfunctional pre-assembly and loading of dynein arms in motile cilia; Authors - Tingting Zhang
mouse	WB	Development; CCDC183 is essential for cytoplasmic invagination around the flagellum during spermiogenesis and male fertility; Authors - Keisuke Shimada
mouse	WB	Exp Anim; The motor domain of testis-enriched kinesin KIF9 is essential for its localization in the mouse flagellum.; Authors - Haruhiko Miyata
human	WB	Ann Hum Genet; DNAH17 is associated with asthenozoospermia and multiple morphological abnormalities of sperm flagella.; Authors - Yanwei Sha

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