Anticorps Polyclonal de lapin anti-DNAI2

DNAI2 Polyclonal Antibody for WB, IHC, IF-P, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 17533-1-PBS

Synonymes

CILD9, Dynein axonemal intermediate chain 2

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Galerie de données de validation

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  WB analysis of mouse ovary using 17533-1-AP (same clone as 17533-1-PBS)

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 17533-1-AP (DNAI2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17533-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse lung using 17533-1-AP (same clone as 17533-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 17533-1-AP (DNAI2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17533-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 17533-1-AP (same clone as 17533-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 17533-1-AP (DNAI2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17533-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse lung using 17533-1-AP (same clone as 17533-1-PBS)

  IP result of anti-DNAI2 (IP:17533-1-AP, 4ug; Detection:17533-1-AP 1:400) with mouse lung tissue lysate 4000ug. This data was developed using the same antibody clone with 17533-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 17533-1-AP (same clone as 17533-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 17533-1-AP (DNAI2 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17533-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse ovary using 17533-1-AP (same clone as 17533-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 17533-1-AP (DNAI2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17533-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse ovary using 17533-1-AP (same clone as 17533-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 17533-1-AP (DNAI2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17533-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 17533-1-AP (same clone as 17533-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using DNAI2 antibody (17533-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17533-1-PBS in a different storage buffer formulation.

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Informations sur le produit

17533-1-PBS cible DNAI2 dans les applications de WB, IHC, IF-P, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DNAI2 Protéine recombinante Ag11585
• Nom complet: dynein, axonemal, intermediate chain 2
• Masse moléculaire calculée: 593 aa, 67 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC039582
• Symbole du gène: DNAI2
• Identification du gène (NCBI): 64446
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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