Anticorps Polyclonal de lapin anti-DNAJA1

• Phare
• Validé par KD/KO

DNAJA1 Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 11713-1-AP

Synonymes

DJ 2, DjA1, DnaJ protein homolog 2, DNAJ2, DNAJA1, hDJ 2, HDJ2, Heat shock 40 kDa protein 4, Heat shock protein J2, HSDJ, HSJ 2, HSJ2, HSPF4, Human DnaJ protein 2

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Galerie de données de validation

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  WB analysis using 11713-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11713-1-AP (DNAJA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11713-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11713-1-AP (DNAJA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11713-1-AP

  WB result of DNAJA1 antibody (11713-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNAJA1 transfected HeLa cells.

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  WB analysis of human testis using 11713-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11713-1-AP (DNAJA1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 11713-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11713-1-AP (DNAJA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 11713-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11713-1-AP (DNAJA1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 11713-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DNAJA1 antibody (11713-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat, tissu hépatique humain, tissu pulmonaire de souris, tissu rénal de rat, tissu rénal de souris, tissu testiculaire humain
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 9 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

11713-1-AP cible DNAJA1 dans les applications de WB, IP, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DNAJA1 Protéine recombinante Ag2284
• Nom complet: DnaJ (Hsp40) homolog, subfamily A, member 1
• Masse moléculaire calculée: 397 aa, 45 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC008182
• Symbole du gène: DNAJA1
• Identification du gène (NCBI): 3301
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DNAJA1 is a member of the DnaJ proteins (also known as Hsp40 or Hsc40) which are cochaperones to DnaK (Hsp70) and function to modulate protein assembly, disassembly, and translocation. DNAJA1 has been reported to get involved in various processes, including virus infection, tau clearance, and mutant p53 degradation.

Publications

Species	Application	Title
human	WB,IF	Nature; A cytosolic surveillance mechanism activates the mitochondrial UPR; Authors - F X Reymond Sutandy ; KD Validated
human	IF	Nat Cell Biol; Heat-shock chaperone HSPB1 regulates cytoplasmic TDP-43 phase separation and liquid-to-gel transition; Authors - Shan Lu
human	WB,IP	Nat Commun; Inhibition of the CDK2 and Cyclin A complex leads to autophagic degradation of CDK2 in cancer cells.; Authors - Jiawei Zhang
human	WB	J Clin Invest; Cul4A-DDB1-mediated monoubiquitination of phosphoglycerate dehydrogenase promotes colorectal cancer metastasis via increased S-adenosylmethionine.; Authors - Yajuan Zhang ; KD Validated
mouse	WB	Mol Ther Nucleic Acids; Age-associated changes in microglia and astrocytes ameliorate blood-brain barrier dysfunction; Authors - Jie Pan
human	WB	Sci Rep; Co-chaperones DNAJA1 and DNAJB6 are critical for regulation of polyglutamine aggregation.; Authors - Claudio Rodríguez-González ; KO Validated