Anticorps Monoclonal anti-DNAJA1

• Phare
• Validé par KD/KO

DNAJA1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgM

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

1B10A10

N° de cat : 67184-1-Ig

Synonymes

HDJ2, hDJ 2, DNAJ2, DjA1, DJ 2

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Galerie de données de validation

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  WB analysis using 67184-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67184-1-Ig (DNAJA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 67184-1-Ig

  WB result of DNAJA1 antibody (67184-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNAJA1 transfected HeLa cells.

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  IHC staining of human placenta using 67184-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67184-1-Ig (DNAJA1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 67184-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67184-1-Ig (DNAJA1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 67184-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67184-1-Ig (DNAJA1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 67184-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67184-1-Ig (DNAJA1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 67184-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67184-1-Ig (DNAJA1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 67184-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67184-1-Ig (DNAJA1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 67184-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DNAJA1 antibody (67184-1-Ig, Clone: 1B10A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules MCF-7, cellules NIH/3T3
• Résultats positifs en IHC: tissu cérébral de souris, tissu cérébral de rat, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67184-1-Ig cible DNAJA1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgM
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DNAJA1 Protéine recombinante Ag2284
• Nom complet: DnaJ (Hsp40) homolog, subfamily A, member 1
• Masse moléculaire calculée: 397 aa, 45 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC008182
• Symbole du gène: DNAJA1
• Identification du gène (NCBI): 3301
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Précipitation des euglobulines
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DNAJA1 is a member of the DnaJ proteins (also known as Hsp40 or Hsc40) which are cochaperones to DnaK (Hsp70) and function to modulate protein assembly, disassembly, and translocation. DNAJA1 has been reported to get involved in various processes, including virus infection, tau clearance, and mutant p53 degradation.

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