Anticorps Monoclonal anti-DNAJA1

• Phare
• Validé par KD/KO

DNAJA1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgM

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B10A10

N° de cat : 67184-1-PBS

Synonymes

HDJ2, hDJ 2, DNAJ2, DjA1, DJ 2

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Galerie de données de validation

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  WB analysis using 67184-1-Ig (same clone as 67184-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67184-1-Ig (DNAJA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67184-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67184-1-Ig (same clone as 67184-1-PBS)

  WB result of DNAJA1 antibody (67184-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNAJA1 transfected HeLa cells. This data was developed using the same antibody clone with 67184-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 67184-1-Ig (same clone as 67184-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67184-1-Ig (DNAJA1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67184-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 67184-1-Ig (same clone as 67184-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67184-1-Ig (DNAJA1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67184-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67184-1-Ig (same clone as 67184-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67184-1-Ig (DNAJA1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67184-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67184-1-Ig (same clone as 67184-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67184-1-Ig (DNAJA1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67184-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 67184-1-Ig (same clone as 67184-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67184-1-Ig (DNAJA1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67184-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 67184-1-Ig (same clone as 67184-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67184-1-Ig (DNAJA1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67184-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67184-1-Ig (same clone as 67184-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DNAJA1 antibody (67184-1-Ig, Clone: 1B10A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67184-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67184-1-PBS cible DNAJA1 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgM
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DNAJA1 Protéine recombinante Ag2284
• Nom complet: DnaJ (Hsp40) homolog, subfamily A, member 1
• Masse moléculaire calculée: 397 aa, 45 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC008182
• Symbole du gène: DNAJA1
• Identification du gène (NCBI): 3301
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Précipitation des euglobulines
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DNAJA1 is a member of the DnaJ proteins (also known as Hsp40 or Hsc40) which are cochaperones to DnaK (Hsp70) and function to modulate protein assembly, disassembly, and translocation. DNAJA1 has been reported to get involved in various processes, including virus infection, tau clearance, and mutant p53 degradation.

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