Anticorps Polyclonal de lapin anti-DNAJA2

DNAJA2 Polyclonal Antibody for IF, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 12236-1-AP

Synonymes

CPR3, DJ3, DJA2, DNAJ, DNAJA2, DNJ3, HIRA interacting protein 4, HIRIP4, PRO3015, RDJ2

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Galerie de données de validation

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  WB analysis of A375 using 12236-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12236-1-AP (DNAJA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12236-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12236-1-AP (DNAJA2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 12236-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12236-1-AP (DNAJA2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of A375 using 12236-1-AP

  IP result of anti-DNAJA2 (IP:12236-1-AP, 4ug; Detection:12236-1-AP 1:300) with A375 cells lysate 3600 ug.

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  IF Staining of MCF-7 using 12236-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 12236-1-AP (DNAJA2 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A375, tissu cérébral humain, tissu rénal de souris
• Résultats positifs en IP: cellules A375,
• Résultats positifs en IF: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below
• IF: See 2 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

12236-1-AP cible DNAJA2 dans les applications de WB, IP, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DNAJA2 Protéine recombinante Ag2877
• Nom complet: DnaJ (Hsp40) homolog, subfamily A, member 2
• Masse moléculaire calculée: 417 aa, 46 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC015809
• Symbole du gène: DNAJA2
• Identification du gène (NCBI): 10294
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Nat Commun; Metamorphism in TDP-43 prion-like domain determines chaperone recognition; Authors - Jaime Carrasco
human	WB	Int J Cancer; Down-regulation of BiP/GRP78 sensitizes resistant prostate cancer cells to gene-therapeutic overexpression of REIC/Dkk-3.; Authors - Tanimoto Ryuta R
human	WB	J Biol Chem; A cytosolic chaperone complex controls folding and degradation of type III CD38.; Authors - Yang Wu
human	WB	Cancers (Basel); Mutant p53 Depletion by Novel Inhibitors for HSP40/J-Domain Proteins Derived from the Natural Compound Plumbagin; Authors - Mohamed Alalem
human,mouse	WB,IP	J Biol Chem; Quantitative interactome proteomics identifies a proteostasis network for GABAA receptors; Authors - Ya-Juan Wang
mouse	WB	Cancer Res; N-myc-Mediated Translation Control Is a Therapeutic Vulnerability in Medulloblastoma; Authors - Duygu Kuzuoglu-Ozturk