Anticorps Monoclonal anti-DNAJB1

DNAJB1 Monoclonal Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1E8E10

N° de cat : 67422-1-PBS

Synonymes

1E8E10, DnaJ protein homolog 1, DNAJ1, hDj 1, Hdj1

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Galerie de données de validation

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  WB analysis using 67422-1-Ig (same clone as 67422-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67422-1-Ig (DNAJB1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67422-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67422-1-Ig (same clone as 67422-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DNAJB1 antibody (67422-1-Ig, Clone: 1E8E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67422-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 67422-1-Ig (same clone as 67422-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human DNAJB1 (67422-1-Ig, Clone:1E8E10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67422-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67422-1-PBS cible DNAJB1 dans les applications de WB, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DNAJB1 Protéine recombinante Ag3830
• Nom complet: DnaJ (Hsp40) homolog, subfamily B, member 1
• Masse moléculaire calculée: 340 aa, 40 kDa
• Poids moléculaire observé: 38 kDa
• Numéro d’acquisition GenBank: BC019827
• Symbole du gène: DNAJB1
• Identification du gène (NCBI): 3337
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DNAJB1 is a member of the heat shock 40 protein family and has been involved in a variety of cellular processes, including the proteasome pathway, endoplasmic reticulum stress and viral infection. DNAJB1-PRKACA gene fusions have been considered diagnostic for fibrolamellar hepatocellular carcinoma.

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