Anticorps Recombinant de lapin anti-DNAJB1

DNAJB1 Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4K3

N° de cat : 82676-1-PBS

Synonymes

hDj-1, Hdj1, hDj 1, DNAJ1, DnaJ protein homolog 1

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Galerie de données de validation

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  WB analysis using 82676-1-RR (same clone as 82676-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82676-1-RR (DNAJB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82676-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse lung using 82676-1-RR (same clone as 82676-1-PBS)

  IP result of anti-DNAJB1 (IP:82676-1-RR, 4ug; Detection:82676-1-RR 1:5000) with mouse lung tissue lysate 1120 ug. This data was developed using the same antibody clone with 82676-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 82676-1-RR (same clone as 82676-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82676-1-RR (DNAJB1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82676-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 82676-1-RR (same clone as 82676-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82676-1-RR (DNAJB1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82676-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 82676-1-RR (same clone as 82676-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DNAJB1 antibody (82676-1-RR, Clone: 4K3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 82676-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82676-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82676-1-RR against Human DNAJB1 were performed. The affinity constant is 0.213 nM.

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Informations sur le produit

82676-1-PBS cible DNAJB1 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: DNAJB1 Protéine recombinante Ag3830
• Nom complet: DnaJ (Hsp40) homolog, subfamily B, member 1
• Masse moléculaire calculée: 340 aa, 40 kDa
• Poids moléculaire observé: 37-40 kDa
• Numéro d’acquisition GenBank: BC019827
• Symbole du gène: DNAJB1
• Identification du gène (NCBI): 3337
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DNAJB1 is a member of the heat shock 40 protein family and has been involved in a variety of cellular processes, including the proteasome pathway, endoplasmic reticulum stress and viral infection. DNAJB1-PRKACA gene fusions have been considered diagnostic for fibrolamellar hepatocellular carcinoma.

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