Anticorps Polyclonal de lapin anti-DNAJB12

• Phare
• Validé par KD/KO

DNAJB12 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, IP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(11)

N° de cat : 16780-1-AP

Synonymes

DJ10

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Galerie de données de validation

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  WB analysis using 16780-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16780-1-AP (DNAJB12 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16780-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16780-1-AP (DNAJB12 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16780-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16780-1-AP (DNAJB12 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 16780-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16780-1-AP (DNAJB12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 16780-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16780-1-AP (DNAJB12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 16780-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 16780-1-AP (DNAJB12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 16780-1-AP

  IP result of anti-DNAJB12 (IP:16780-1-AP, 4ug; Detection:16780-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human stomach using 16780-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16780-1-AP (DNAJB12 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 16780-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16780-1-AP (DNAJB12 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 16780-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16780-1-AP (DNAJB12 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 16780-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16780-1-AP (DNAJB12 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules A549, cellules MCF-7, cellules MKN-45, tissu cérébral de rat, tissu cérébral humain, tissu hépatique humain, tissu rénal humain
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu d'estomac humain, human ovary cancer tissue; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IF: See 3 publications below
• IP: See 3 publications below
• ChIP: See 1 publications below

Informations sur le produit

16780-1-AP cible DNAJB12 dans les applications de WB, IHC, IF, IP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, singe
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DNAJB12 Protéine recombinante Ag10193
• Nom complet: DnaJ (Hsp40) homolog, subfamily B, member 12
• Masse moléculaire calculée: 375 aa, 42 kDa
• Poids moléculaire observé: 40-42 kDa
• Numéro d’acquisition GenBank: BC011812
• Symbole du gène: DNAJB12
• Identification du gène (NCBI): 54788
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DNAJB12 (JB12), a ER transmembrane chaperone, specifically interacts with Hsc70 to facilitate ER retention and delivery of membrane proteins for folding or degradation in the ERAD pathway. Hsc70 chaperone activities require the substrate targeting function of DNAJB12. DNAJB12 recruits specific substrates to contact Hsc70 and then stimulates its ATPase activity. (PMID: 34668642)

Publications

Species	Application	Title
human	WB,ChIP	Mol Cell; An oncogenic JMJD6-DGAT1 axis tunes the epigenetic regulation of lipid droplet formation in clear cell renal cell carcinoma.; Authors - Jin Zhou
human	IP	Nat Commun; The methyltransferase METTL9 mediates pervasive 1-methylhistidine modification in mammalian proteomes.; Authors - Erna Davydova
	WB, IP	J Cell Biol; Reticulon protects the integrity of the ER membrane during ER escape of large macromolecular protein complexes.; Authors - Yu-Jie Chen
human	WB	PLoS Pathog; A cytosolic chaperone complexes with dynamic membrane J-proteins and mobilizes a nonenveloped virus out of the endoplasmic reticulum.; Authors - Christopher Paul Walczak
human	WB,IF	J Cell Biol; Reticulons promote formation of ER-derived double-membrane vesicles that facilitate SARS-CoV-2 replication; Authors - Jeffrey M Williams
human	IF	J Virol; The ER membrane J protein C18 executes a distinct role in promoting SV40 membrane penetration.; Authors - Parikshit Bagchi

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