Anticorps Polyclonal de lapin anti-DNAJB14

DNAJB14 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 16501-1-PBS

Synonymes

UNQ9427/PRO34683, PRO34683, EGNR9427

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Galerie de données de validation

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  WB analysis of Jurkat using 16501-1-AP (same clone as 16501-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16501-1-AP (DNAJB14 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16501-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 16501-1-AP (same clone as 16501-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16501-1-AP (DNAJB14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16501-1-PBS in a different storage buffer formulation.

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  WB analysis of human testis using 16501-1-AP (same clone as 16501-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 16501-1-AP (DNAJB14 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16501-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 16501-1-AP (same clone as 16501-1-PBS)

  IP result of anti-DNAJB14 (IP:16501-1-AP, 4ug; Detection:16501-1-AP 1:1000) with Jurkat cells lysate 3080ug. This data was developed using the same antibody clone with 16501-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 16501-1-AP (same clone as 16501-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16501-1-AP (DNAJB14 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16501-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 16501-1-AP (same clone as 16501-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using DNAJB14 antibody (16501-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 16501-1-PBS in a different storage buffer formulation.

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Informations sur le produit

16501-1-PBS cible DNAJB14 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DNAJB14 Protéine recombinante Ag9668
• Nom complet: DnaJ (Hsp40) homolog, subfamily B, member 14
• Masse moléculaire calculée: 379 aa, 43 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC022248
• Symbole du gène: DNAJB14
• Identification du gène (NCBI): 79982
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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