Anticorps Polyclonal de lapin anti-DNAJB2

DNAJB2 Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 10838-1-AP

Synonymes

DnaJ protein homolog 1, DNAJB2, Heat shock 40 kDa protein 3, Heat shock protein J1, HSJ 1, HSJ1, HSPF3

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Galerie de données de validation

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  WB analysis using 10838-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10838-1-AP (DNAJB2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10838-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10838-1-AP (DNAJB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 10838-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10838-1-AP (DNAJB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10838-1-AP

  IP Result of anti-DNAJB2 (IP:10838-1-AP, 4ug; Detection:10838-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, cellules A431, tissu cardiaque de rat, tissu cérébral de rat, tissu cérébral de souris, tissu de muscle squelettique de souris
• Résultats positifs en IP: tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

10838-1-AP cible DNAJB2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DNAJB2 Protéine recombinante Ag1271
• Nom complet: DnaJ (Hsp40) homolog, subfamily B, member 2
• Masse moléculaire calculée: 36 kDa
• Poids moléculaire observé: 36-40 kDa
• Numéro d’acquisition GenBank: BC011609
• Symbole du gène: DNAJB2
• Identification du gène (NCBI): 3300
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The DNAJB2 (also known as HSJ1a, Heat-Shock Protein J1a) is a member of the DNAJ family of molecular chaperones which play important cellular roles in correct protein folding, in the response to protein misfolding, and in the degradation of misfolded proteins. It is expressed principally in the nervous system and is a key player in neuronal proteostasis maintenance. DNAJB2 has been proposed as a potent anti-aggregation chaperone for TDP-43, may play a role in the pathogenesis of ALS. DNAJB2 gene encodes two alternatively-spliced isoforms that differ in their C-terminus. The variant 1 form (V1 or HSJ1a) is expressed in both cytoplasm and nucleus, whereas the variant 2 form (V2 or HSJ1b) undergoes post-translational geranylgeranylation modification which mediates its attachment to the cytoplasmic side of the endoplasmic reticulum membrane. This antibody detects both isoforms of DNAJB2 with MW between 32-40 kDa. (PMID: 20395441)

Publications

Species	Application	Title
human	WB, IF	Ann Neurol; A rare recessive distal hereditary motor neuropathy with HSJ1 chaperone mutation.; Authors - Blumen Sergiu C SC
		Diabetes; PMID: 24379352; Authors - Brian G Drew
human	WB,IHC	Eur J Neurol; DNAJB2-related Charcot-Marie-Tooth disease type 2: Pathomechanism insights and phenotypic spectrum widening.; Authors - Paola Saveri
human,mouse	WB,IHC	Am J Pathol; DNAJB2 expression in normal and diseased human and mouse skeletal muscle.; Authors - Claeys Kristl G KG
human	WB,IF	Sci Rep; PolyQ-expanded proteins impair cellular proteostasis of ataxin-3 through sequestering the co-chaperone HSJ1 into aggregates.; Authors - Hong-Wei Yue
human	WB	Cancers (Basel); Mutant p53 Depletion by Novel Inhibitors for HSP40/J-Domain Proteins Derived from the Natural Compound Plumbagin; Authors - Mohamed Alalem