Anticorps Polyclonal de lapin anti-DNAJB3

• Phare
• Validé par KD/KO

DNAJB3 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 17177-1-AP

Synonymes

DNAJB3, HCG3

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Galerie de données de validation

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  WB analysis of mouse testis using 17177-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17177-1-AP (DNAJB3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 17177-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17177-1-AP (DNAJB3 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17177-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17177-1-AP (DNAJB3 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 17177-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 17177-1-AP (DNAJB3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 17177-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17177-1-AP (DNAJB3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 17177-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17177-1-AP (DNAJB3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 17177-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17177-1-AP (DNAJB3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 17177-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17177-1-AP (DNAJB3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 17177-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17177-1-AP (DNAJB3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 17177-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17177-1-AP (DNAJB3 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, tissu testiculaire de rat
• Résultats positifs en IHC: tissu testiculaire de souris, tissu ovarien humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 2 publications below

Informations sur le produit

17177-1-AP cible DNAJB3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DNAJB3 Protéine recombinante Ag10878
• Nom complet: DnaJ (Hsp40) homolog, subfamily B, member 3
• Masse moléculaire calculée: 145 aa, 17 kDa
• Poids moléculaire observé: 28-30 kDa
• Numéro d’acquisition GenBank: BC024013
• Symbole du gène: DNAJB3
• Identification du gène (NCBI): 414061
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Sci Rep; PMID: 26400768; Authors - Mohamed Abu-Farha
mouse	WB	Sci Rep; Alpha lipoic acid attenuates ER stress and improves glucose uptake through DNAJB3 cochaperone.; Authors - Abdoulaye Diane ; KD Validated
human	WB,IHC	PLoS One; DNAJB3/HSP-40 Cochaperone Is Downregulated in Obese Humans and Is Restored by Physical Exercise.; Authors - Abubaker Jehad J
mouse	WB	Int J Mol Sci; Expression of Human DNAJ (Heat Shock Protein-40) B3 in Humanized UDP-glucuronosyltransferase 1 Mice.; Authors - Ryo Mitsugi
human	WB,IHC	Lipids Health Dis; Immunohistochemical profiling of the heat shock response in obese non-diabetic subjects revealed impaired expression of heat shock proteins in the adipose tissue.; Authors - Ali Tiss
mouse	WB	Sci Rep; DNAJB3 attenuates metabolic stress and promotes glucose uptake by eliciting Glut4 translocation.; Authors - Abdelilah Arredouani ; KD Validated