Anticorps Polyclonal de lapin anti-DNAJB8

DNAJB8 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 17071-1-AP

Synonymes

DJ6

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Galerie de données de validation

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  WB analysis using 17071-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17071-1-AP (DNAJB8 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17071-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17071-1-AP (DNAJB8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 17071-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17071-1-AP (DNAJB8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 17071-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17071-1-AP (DNAJB8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain
• Résultats positifs en IHC: tissu testiculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

17071-1-AP cible DNAJB8 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DNAJB8 Protéine recombinante Ag10899
• Nom complet: DnaJ (Hsp40) homolog, subfamily B, member 8
• Masse moléculaire calculée: 232 aa, 26 kDa
• Poids moléculaire observé: 26 kDa
• Numéro d’acquisition GenBank: BC029521
• Symbole du gène: DNAJB8
• Identification du gène (NCBI): 165721
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DNAJB8, a member of the DNAJ/HSP40 family of proteins, plays a crucial role in regulating chaperone HSP70 activity. It is known for its ability to suppress the aggregation of polyglutamine proteins through its C-terminal tail. Furthermore, DNAJB8 has been shown to inhibit TP53 through ubiquitin degradation in human colon cancer by directly interacting and upregulating MDR1, leading to increased oxaliplatin resistance. High levels of DNAJB8 in both colon tissues and serum are correlated with worse overall survival of colon cancer patients, suggesting that DNAJB8 level in serum sEV may serve as a potential biomarker for colon cancer and a promising therapeutic target for oxaliplatin-resistant cancer.

Publications

Species	Application	Title
mouse	WB,IF	iScience; Proteostasis regulated by testis-specific ribosomal protein RPL39L maintains mouse spermatogenesis.; Authors - Qianxing Zou
mouse	WB,IF	STAR Protoc; Protocol for isolation and proteostatic analysis of sub-populations of spermatogenic cells in mouse.; Authors - Qianxing Zou
pig	WB	Vet Microbiol; The heat shock protein DNAJB8 inhibits pseudorabies virus replication by autophagy; Authors - Xiaoyong Chen

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