Anticorps Polyclonal de lapin anti-DNAJC11

DNAJC11 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 17331-1-AP

Synonymes

DNAJC11

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Galerie de données de validation

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  WB analysis using 17331-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17331-1-AP (DNAJC11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17331-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17331-1-AP (DNAJC11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17331-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17331-1-AP (DNAJC11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 17331-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17331-1-AP (DNAJC11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human skeletal muscle using 17331-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 17331-1-AP (DNAJC11 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 17331-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17331-1-AP (DNAJC11 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 17331-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17331-1-AP (DNAJC11 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 17331-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17331-1-AP (DNAJC11 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse brain using 17331-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17331-1-AP (DNAJC11 Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules HepG2, cellules SH-SY5Y, tissu hépatique de souris
• Résultats positifs en IHC: tissu hépatique humain, tissu cérébral de souris, tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

17331-1-AP cible DNAJC11 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DNAJC11 Protéine recombinante Ag11310
• Nom complet: DnaJ (Hsp40) homolog, subfamily C, member 11
• Masse moléculaire calculée: 507 aa, 57 kDa
• Poids moléculaire observé: 63 kDa
• Numéro d’acquisition GenBank: BC014145
• Symbole du gène: DNAJC11
• Identification du gène (NCBI): 55735
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DNAJC11 is a member of the J proteins also known as HSP40 family of co-chaperones. Recently DNAJC11 has been found to interact with mitofilin, a mitochondrial inner membrane protein, indicating that it may be a molecular chaperone during mitochondrial protein import and folding.

Publications

Species	Application	Title
human	WB	J Proteome Res; Mapping Interactome Networks of DNAJC11, a Novel Mitochondrial Protein Causing Neuromuscular Pathology in Mice.; Authors - Foteini Violitzi
mouse	WB	PLoS One; A Splicing Mutation in the Novel Mitochondrial Protein DNAJC11 Causes Motor Neuron Pathology Associated with Cristae Disorganization, and Lymphoid Abnormalities in Mice.; Authors - Fotis Ioakeimidis
human,mouse	WB	Nat Cell Biol; Small heat shock proteins operate as molecular chaperones in the mitochondrial intermembrane space; Authors - Elias Adriaenssens