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Anticorps Polyclonal de lapin anti-DNAJC13

DNAJC13 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 31492-1-PBS

Synonymes

RME8, RME 8, KIAA0678

Galerie de données de validation

Filter:
  • Western Blot (WB) analysis of various lysates using DNAJC13 Polyclonal antibody (31492-1-AP)

    WB analysis using 31492-1-AP (same clone as 31492-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31492-1-AP (DNAJC13 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31492-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using DNAJC13 Polyclonal antibody (31492-1-AP)

    IF Staining of HeLa using 31492-1-AP (same clone as 31492-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using DNAJC13 antibody (31492-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 31492-1-PBS in a different storage buffer formulation.

Informations sur le produit

31492-1-PBS cible DNAJC13 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène DNAJC13 Protéine recombinante Ag35412
Nom complet DnaJ (Hsp40) homolog, subfamily C, member 13
Poids moléculaire observé254 kDa
Numéro d’acquisition GenBankBC043583
Symbole du gène DNAJC13
Identification du gène (NCBI) 23317
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DNAJC13 is a large protein with 2,243 amino acids. Due to the presence of the central DNAJ domain, DNAJC13 binds HSC70 (heat shock cognate 70) and acts as a co-chaperone in mediating HSC70 cellular functions. Within the cell, DNAJC13 is primarily localized at membranous structures. This interaction is mediated, at least in part, by the binding to phosphoinositides (PI) through its N-terminal sequence. RME-8/DNAJC13 is involved in several endosomal functions such as protein sorting, endosomal tubulation, transport processes including the retrograde transport from the endosome to the trans-Golgi network (TGN), the formation of endosomal degradative microdomains, and the recycling of membrane receptors. (PMID: 32322926)

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