Anticorps Polyclonal de lapin anti-DNAJC18

• Phare
• Validé par KD/KO

DNAJC18 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 25162-1-PBS

Synonymes

DNAJC 18

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Galerie de données de validation

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  WB analysis of A431 using 25162-1-AP (same clone as 25162-1-PBS)

  WB result of DNAJC18 antibody (25162-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNAJC18 transfected A431 cells. This data was developed using the same antibody clone with 25162-1-PBS in a different storage buffer formulation.

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  WB analysis using 25162-1-AP (same clone as 25162-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25162-1-AP (DNAJC18 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25162-1-PBS in a different storage buffer formulation.

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  WB analysis using 25162-1-AP (same clone as 25162-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25162-1-AP (DNAJC18 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25162-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 25162-1-AP (same clone as 25162-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25162-1-AP (DNAJC18 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25162-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 25162-1-AP (same clone as 25162-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25162-1-AP (DNAJC18 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25162-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 25162-1-AP (same clone as 25162-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using DNAJC18 antibody (25162-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25162-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 25162-1-AP (same clone as 25162-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using 25162-1-AP (DNAJC18 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25162-1-PBS in a different storage buffer formulation.

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Informations sur le produit

25162-1-PBS cible DNAJC18 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DNAJC18 Protéine recombinante Ag18498
• Nom complet: DnaJ (Hsp40) homolog, subfamily C, member 18
• Masse moléculaire calculée: 358 aa, 42 kDa
• Poids moléculaire observé: 39-42 kDa
• Numéro d’acquisition GenBank: BC030162
• Symbole du gène: DNAJC18
• Identification du gène (NCBI): 202052
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DnaJC18, also named as DnaJ (Hsp40) homolog, subfamily C, member 21, belongs to Type III DnaJ Family. Type III DnaJ proteins have a J domain but lack the other sequence features that are found in type I and II members of the family. DnaJC18 might play a role in the process of spermatogenesis in adult testis (PMID: 29082339).

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