Anticorps Polyclonal de lapin anti-DNAJC19

• Phare
• Validé par KD/KO

DNAJC19 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12096-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HeLa using 12096-1-AP (same clone as 12096-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12096-1-AP (DNAJC19 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12096-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 12096-1-AP (same clone as 12096-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 12096-1-AP (DNAJC19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12096-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 12096-1-AP (same clone as 12096-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12096-1-AP (DNAJC19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12096-1-PBS in a different storage buffer formulation.

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  WB analysis of human lung using 12096-1-AP (same clone as 12096-1-PBS)

  human lung tissue were subjected to SDS PAGE followed by western blot with 12096-1-AP (DNAJC19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12096-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse heart using 12096-1-AP (same clone as 12096-1-PBS)

  IP result of anti-DNAJC19 (IP:12096-1-AP, 4ug; Detection:12096-1-AP 1:1000) with mouse heart tissue lysate 4000ug. This data was developed using the same antibody clone with 12096-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 12096-1-AP (same clone as 12096-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using DNAJC19 antibody (12096-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12096-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12096-1-PBS cible DNAJC19 dans les applications de WB, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DNAJC19 Protéine recombinante Ag2739
• Nom complet: DnaJ (Hsp40) homolog, subfamily C, member 19
• Masse moléculaire calculée: 116 aa, 13 kDa
• Poids moléculaire observé: 13 kDa
• Numéro d’acquisition GenBank: BC009702
• Symbole du gène: DNAJC19
• Identification du gène (NCBI): 131118
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DNAJC19 is a mitochondrial cochaperone that interacts with HSP70 chaperones through its conserved J-domain. As a transmembrane protein, DNAJC19 is strongly associated with the inner mitochondrial membrane. Mutations in DNAJC19 cause dilated cardiomyopathy with ataxia (DCMA). Recently DNAJC19 has been reported as interactor of PHB complex to regulate cardiolipin remodeling, which addressed the link of DNAJC19 to cardiomyopathy. This antibody is specific to DNAJC19 and had been tested by siRNA.(24856930)

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