Anticorps Polyclonal de lapin anti-DNAJC2/MPP11

DNAJC2/MPP11 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 11971-1-AP

Synonymes

DNAJC2, M phase phosphoprotein 11, MPP11, ZRF1, ZUO1, Zuotin related factor 1

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Galerie de données de validation

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  WB analysis of K-562 using 11971-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 11971-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 11971-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11971-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11971-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11971-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11971-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11971-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11971-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11971-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 11971-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 11971-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11971-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 11971-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11971-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11971-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11971-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 11971-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11971-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11971-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11971-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11971-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 11971-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11971-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11971-1-AP (DNAJC2/MPP11 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 11971-1-AP

  IP Result of anti-DNAJC2/MPP11 (IP:11971-1-AP, 4ug; Detection:11971-1-AP 1:1000) with mouse liver tissue lysate 6000ug.

• 

  IHC staining of human liver cancer using 11971-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11971-1-AP (DNAJC2/MPP11 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11971-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11971-1-AP (DNAJC2/MPP11 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11971-1-AP

  Immunofluorescent analysis of HepG2 cells, using DNAJC2 antibody 11971-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules HEK-293, cellules HeLa, cellules HepG2, cellules PC-3, tissu hépatique de souris
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

11971-1-AP cible DNAJC2/MPP11 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DNAJC2/MPP11 Protéine recombinante Ag2594
• Nom complet: DnaJ (Hsp40) homolog, subfamily C, member 2
• Masse moléculaire calculée: 72 kDa
• Poids moléculaire observé: 66-75 kDa
• Numéro d’acquisition GenBank: BC056682
• Symbole du gène: DNAJC2
• Identification du gène (NCBI): 27000
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	EMBO J; Dual roles of HSP70 chaperone HSPA1 in quality control of nascent and newly synthesized proteins.; Authors - Guiyou Tian
human	WB	Cancers (Basel); Mutant p53 Depletion by Novel Inhibitors for HSP40/J-Domain Proteins Derived from the Natural Compound Plumbagin; Authors - Mohamed Alalem