Anticorps Polyclonal de lapin anti-DNAJC3

DNAJC3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 26721-1-PBS

Synonymes

---

Galerie de données de validation

• 

  WB analysis using 26721-1-AP (same clone as 26721-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 26721-1-AP (DNAJC3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26721-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 26721-1-AP (same clone as 26721-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26721-1-AP (DNAJC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26721-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 26721-1-AP (same clone as 26721-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26721-1-AP (DNAJC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26721-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 26721-1-AP (same clone as 26721-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26721-1-AP (DNAJC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26721-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 26721-1-AP (same clone as 26721-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26721-1-AP (DNAJC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26721-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U2OS using 26721-1-AP (same clone as 26721-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using 26721-1-AP (DNAJC3 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26721-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

26721-1-PBS cible DNAJC3 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DNAJC3 Protéine recombinante Ag24966
• Nom complet: DnaJ (Hsp40) homolog, subfamily C, member 3
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: BC047936
• Symbole du gène: DNAJC3
• Identification du gène (NCBI): 5611
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DNAJC3, also known as P58,HP58 and P58IPK, belongs to heat shock protein family,which is a kind of conservative molecules that expresses extensively in cells of human and animals and protects cells against all kinds of damage. As a cochaperone of BiP that an HSP70 molecular chaperone located in the lumen of the endoplasmic reticulum(ER), DNAJC3 plays a crucial role in ER protein folding and the UPR(unfolded protein response) (PMID:10597629; PMID:25466870). Studies reported that DNAJC3 related several diseases such as osteosarcoma, widespread neurodegeneration, diabetes mellitus, sensorimotor neuropathy, ataxia, sensorineural hearing loss and short body stature(PMID:30652414; PMID:28940199).

{{ptg:RelatedPrimaryAntibodies}}