Anticorps Polyclonal de lapin anti-DNAJC5

DNAJC5 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 32204-1-PBS

Synonymes

Ceroid-lipofuscinosis neuronal protein 4, CLN4, CSP, Cysteine string protein, DnaJ (Hsp40) Homolog, Subfamily C, Member 5

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Galerie de données de validation

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  WB analysis using 32204-1-AP (same clone as 32204-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 32204-1-AP (DNAJC5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32204-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 32204-1-AP (same clone as 32204-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 32204-1-AP (DNAJC5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32204-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 32204-1-AP (same clone as 32204-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 32204-1-AP (DNAJC5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32204-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 32204-1-AP (same clone as 32204-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 32204-1-AP (DNAJC5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32204-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 32204-1-AP (same clone as 32204-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 32204-1-AP (DNAJC5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32204-1-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 32204-1-AP (same clone as 32204-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 32204-1-AP (DNAJC5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32204-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 32204-1-AP (same clone as 32204-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 32204-1-AP (DNAJC5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32204-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 32204-1-AP (same clone as 32204-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using DNAJC5 antibody (32204-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 32204-1-PBS in a different storage buffer formulation.

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Informations sur le produit

32204-1-PBS cible DNAJC5 dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DNAJC5 Protéine recombinante Ag35443
• Nom complet: DnaJ (Hsp40) homolog, subfamily C, member 5
• Masse moléculaire calculée: 22kDa，198aa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: NM_025219.2
• Symbole du gène: DNAJC5
• Identification du gène (NCBI): 80331
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DNAJC5 (DnaJ homolog subfamily C member 5), also known as cysteine string protein α (CSPα), is a member of the HSP40 (DNAJ) family of proteins, as well as a co-chaperone of HSC70, and has been shown to control the extracellular release of many neurodegenerative disease proteins. DNAJC5 contains three structural domains - -a common N-terminal J structural domain conserved in DNAJ proteins, the cysteine string (CS) central structural domain, which is highly palmitoylated and anchors the protein to late endosomes, and an overall disordered C-terminal structural domain. Mutations in DNAJC5 have been strongly implicated in the development of human diseases such as neuronal waxy lipofuscinosis, cystic fibrosis, Huntington's disease and Parkinson's disease.

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