Anticorps Polyclonal de lapin anti-DNAJC9

• Phare
• Validé par KD/KO

DNAJC9 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

1

Avis (2)

N° de cat : 25444-1-AP

Synonymes

DnaJ protein SB73, DNAJC9, HDJC9, JDD1, KIAA0974, SB73

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Galerie de données de validation

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  WB analysis of HEK-293 using 25444-1-AP

  WB result of DNAJC9 antibody (25444-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DNAJC9 transfected HEK-293 cells.

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  WB analysis using 25444-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25444-1-AP (DNAJC9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 25444-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 25444-1-AP (DNAJC9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 25444-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25444-1-AP (DNAJC9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 25444-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25444-1-AP (DNAJC9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 25444-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25444-1-AP (DNAJC9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 25444-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25444-1-AP (DNAJC9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 25444-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 25444-1-AP (DNAJC9 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 25444-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 25444-1-AP (DNAJC9 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat colon using 25444-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 25444-1-AP (DNAJC9 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat colon using 25444-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 25444-1-AP (DNAJC9 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 25444-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25444-1-AP (DNAJC9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 25444-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25444-1-AP (DNAJC9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 25444-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using DNAJC9 antibody (25444-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules Raji, cellules SH-SY5Y
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du côlon humain, tissu de côlon de rat, tissu de côlon de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

25444-1-AP cible DNAJC9 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DNAJC9 Protéine recombinante Ag22123
• Nom complet: DnaJ (Hsp40) homolog, subfamily C, member 9
• Masse moléculaire calculée: 260 aa, 30 kDa
• Poids moléculaire observé: 30-35 kDa
• Numéro d’acquisition GenBank: BC136507
• Symbole du gène: DNAJC9
• Identification du gène (NCBI): 23234
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DNAJC9, DnaJ homolog subfamily C member 9, acts as a dual histone chaperone and heat shock co-chaperone (PMID: 33857403). As a histone chaperone, DNAJC9 forms a co-chaperone complex with MCM2 and histone H3-H4 heterodimers (PMID: 33857403). DNAJC9 also plays a role as co-chaperone of the HSP70 family of molecular chaperone proteins. (PMID: 17182002, PMID: 33857403). DNAJC9 exhibits activity to assemble histones onto DNA (PMID: 33857403). DNAJC9 is an essential protein in many cancer cell types and the levels of the protein correlate with the rates at which cancer cells proliferate.

Publications

Species	Application	Title
human	WB	Sci Rep; Label-free Quantitative Analysis of Protein Expression Alterations in miR-26a-Knockout HeLa Cells using SWATH-MS Technology.; Authors - Hexiao Shen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

HELENA (Verified Customer) (04-26-2023)

Samples correspond to activated human primary CD4+ T cells after knock-out of DNAJC9 by CRISPR/CAS9.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human primary CD4+ T cells

DNAJC9 Antibody Western Blot validation (1:1000 dilution) in Human primary CD4+ T cells (Cat no:25444-1-AP)

FH

Sven (Verified Customer) (11-27-2021)

Works very well on western blot for human and mouse tissue (1:5000 dilution in TBS-T with 50% glycerol, 2% BSA, 0.02% NaN3). Antiboydy specificity was validated by DNAJC9 knockout cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Human + Mouse pancreatic cancer cell lines

DNAJC9 Antibody Western Blot validation (1:5000 dilution) in Human + Mouse pancreatic cancer cell lines (Cat no:25444-1-AP)