Anticorps Polyclonal de lapin anti-DNALI1

DNALI1 Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 17601-1-AP

Synonymes

DNALI1, hp28, P28

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Galerie de données de validation

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  WB analysis using 17601-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17601-1-AP (DNALI1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 17601-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17601-1-AP (DNALI1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 17601-1-AP

  IP Result of anti-DNALI1 (IP:17601-1-AP, 4ug; Detection:17601-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, tissu testiculaire de rat
• Résultats positifs en IP: tissu testiculaire de souris

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IF: See 4 publications below
• CoIP: See 1 publications below

Informations sur le produit

17601-1-AP cible DNALI1 dans les applications de WB, IP, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DNALI1 Protéine recombinante Ag11652
• Nom complet: dynein, axonemal, light intermediate chain 1
• Masse moléculaire calculée: 258 aa, 30 kDa
• Poids moléculaire observé: 30-33 kDa
• Numéro d’acquisition GenBank: BC046117
• Symbole du gène: DNALI1
• Identification du gène (NCBI): 7802
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DNALI1 belongs to the inner dynein arm light chain family and encodes a subunit of the light intermediate chain of inner dynein arms of the axoneme of sperm flagella (PMID: 36792588). DNALI1 promotes neurodegeneration after traumatic brain injury via inhibition of autophagosome-lysosome fusion (PMID: 38348540). Mutation in DNALI1 Causes Male Infertility with Severe Oligoasthenoteratozoospermia in Humans (PMID: 38212584).

Publications

Species	Application	Title
rat	WB,IF	Front Cell Dev Biol; PIH1D3-knockout rats exhibit full ciliopathy features and dysfunctional pre-assembly and loading of dynein arms in motile cilia; Authors - Tingting Zhang
mouse	WB	Development; CCDC183 is essential for cytoplasmic invagination around the flagellum during spermiogenesis and male fertility; Authors - Keisuke Shimada
human	IF	Clin Genet; DNAH2 is a novel candidate gene associated with multiple morphological abnormalities of the sperm flagella.; Authors - Yang Li
mouse	WB,IF	Basic Clin Androl; Dnali1 is required for sperm motility and male fertility in mice; Authors - Yiling Zhou
mouse	WB	Exp Anim; The motor domain of testis-enriched kinesin KIF9 is essential for its localization in the mouse flagellum.; Authors - Haruhiko Miyata
mouse	WB,IF,CoIP	Elife; DNALI1 interacts with the MEIG1/PACRG complex within the manchette and is required for proper sperm flagellum assembly in mice; Authors - Yi Tian Yap ; KO Validated