Anticorps Recombinant de lapin anti-DNase I

DNase I Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

240222F11

N° de cat : 83337-6-RR

Synonymes

DNASE1, EC:3.1.21.1, DRNI, DNL1, Deoxyribonuclease-1

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Galerie de données de validation

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  WB analysis of mouse heart using 83337-6-RR

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 83337-6-RR (DNase I antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human urine using 83337-6-RR

  human urine sample were subjected to SDS PAGE followed by western blot with 83337-6-RR (DNase I antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, échantillon d'urine humaine

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83337-6-RR cible DNase I dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: DNase I Protéine recombinante Ag10117
• Nom complet: deoxyribonuclease I
• Masse moléculaire calculée: 282 aa, 31 kDa
• Poids moléculaire observé: 31-40 kDa
• Numéro d’acquisition GenBank: BC029437
• Symbole du gène: DNASE1
• Identification du gène (NCBI): 1773
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Deoxyribonuclease I (DNase I), also known as DNL1 and DRNI, belongs to the DNase I family. DNase I is a DNA-digestive glycoprotein that cleaves both single- and double stranded DNA through hydrolysis of the phosphodiester bond. In humans, the DNase I enzyme is primarily secreted by the pancreas, parotid, and pituitary glands into the digestive system and bloodstream. Since DNase I is ubiquitously expressed in human tissues, it is thought to play a similar anti-autoimmune role in other organs as well (PMID: 21806320). The calculated molecular weight of DNase I is 31 kDa. Sometimes higher molecular weight around 40 kDa can also be observed, which is caused by glycosylation (PMID: 34329318).

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