Anticorps Monoclonal anti-DNASE1L3

DNASE1L3 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

1A4D4

N° de cat : 67041-1-PBS

Synonymes

1A4D4, Deoxyribonuclease gamma, Deoxyribonuclease I-like 3, DHP2, DNAS1L3

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Galerie de données de validation

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  WB analysis using 67041-1-Ig (same clone as 67041-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67041-1-Ig (DNASE1L3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67041-1-PBS in a different storage buffer formulation.

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  WB analysis using 67041-1-Ig (same clone as 67041-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67041-1-Ig (DNASE1L3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67041-1-PBS in a different storage buffer formulation.

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  WB analysis of Daudi using 67041-1-Ig (same clone as 67041-1-PBS)

  Daudi cells were subjected to SDS PAGE followed by western blot with 67041-1-Ig (DNASE1L3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67041-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 67041-1-Ig (same clone as 67041-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67041-1-Ig (DNASE1L3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67041-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 67041-1-Ig (same clone as 67041-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67041-1-Ig (DNASE1L3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67041-1-PBS in a different storage buffer formulation.

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  IHC staining of human spleen using 67041-1-Ig (same clone as 67041-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67041-1-Ig (DNASE1L3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67041-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 67041-1-Ig (same clone as 67041-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67041-1-Ig (DNASE1L3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67041-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67041-1-PBS cible DNASE1L3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DNASE1L3 Protéine recombinante Ag28187
• Nom complet: deoxyribonuclease I-like 3
• Masse moléculaire calculée: 305 aa, 36 kDa
• Poids moléculaire observé: 33-38 kDa
• Numéro d’acquisition GenBank: BC015831
• Symbole du gène: DNASE1L3
• Identification du gène (NCBI): 1776
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Deoxyribonuclease 1-like 3 (DNASE1L3, DNase gamma, DNase Y, LS-DNase) is member of a DNASE1 protein family that is identified by similar biochemical properties such as Ca2+/Mg2+- dependency and an optimal pH of about 7.0 as well as by a high similarity in their nucleic acid and amino acid sequences (PMID:157967140). It is an endonuclease and cleaves double-stranded DNA (and single-stranded DNA) producing 3′-OH/5′-P ends, which is Ca2+/Mg2+-dependent and inhibited by Zn2+, but not by monomeric actin (PMID:9665719). In addition, it translocates from the endoplasmic reticulum to the nucleus during apoptosis (PMID: 23229555). DNASE1L3 can be located either in the cytoplasm or in the nucleus.

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