Anticorps Polyclonal de lapin anti-Dynamin 1

• Phare
• Validé par KD/KO

Dynamin 1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(9)

N° de cat : 18205-1-AP

Synonymes

DNM, DNM1, dynamin 1, Dynamin1

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Galerie de données de validation

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  WB analysis of mouse brain using 18205-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 18205-1-AP (Dynamin 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 18205-1-AP

  WB result of Dynamin 1 antibody (18205-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Dynamin 1 transfected A549 cells.

• 

  WB analysis of NIH/3T3 using 18205-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18205-1-AP (Dynamin 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 18205-1-AP

  IP result of anti-Dynamin 1 (IP:18205-1-AP, 4ug; Detection:18205-1-AP 1:2000) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of rat brain using 18205-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18205-1-AP (Dynamin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 18205-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 18205-1-AP (Dynamin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 18205-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18205-1-AP (Dynamin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 18205-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18205-1-AP (Dynamin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of Neuro-2a using 18205-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using 18205-1-AP (Dynamin 1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules A549, cellules NIH/3T3
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de rat, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules Neuro-2a,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IF: See 1 publications below

Informations sur le produit

18205-1-AP cible Dynamin 1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, poulet, souris, fish
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Dynamin 1 Protéine recombinante Ag12827
• Nom complet: dynamin 1
• Masse moléculaire calculée: 851 aa, 96 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC063850
• Symbole du gène: DNM1
• Identification du gène (NCBI): 1759
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Elife; Amphiphysin I cleavage by asparagine endopeptidase leads to tau hyperphosphorylation and synaptic dysfunction.; Authors - Xingyu Zhang
mouse,pig	WB	PLoS Pathog; Pseudorabies virus inhibits progesterone-induced inactivation of TRPML1 to facilitate viral entry; Authors - Bing-Qian Su
fish	WB	Int J Mol Sci; Dietary Nano-ZnO Is Absorbed via Endocytosis and ZIP Pathways, Upregulates Lipogenesis, and Induces Lipotoxicity in the Intestine of Yellow Catfish.; Authors - Shu-Wei Chen
human	WB	Commun Biol; The germline factor DDX4 contributes to the chemoresistance of small cell lung cancer cells; Authors - Christopher Noyes ; KD Validated
human	WB	Neurobiol Dis; Asparagine endopeptidase cleaves synaptojanin 1 and triggers synaptic dysfunction in Parkinson's disease.; Authors - Li Zou
mouse	IF	J Neurosci; The Structural and Functional Integrity of Rod Photoreceptor Ribbon Synapses Depends on Redundant Actions of Dynamins 1 and 3; Authors - Christin Hanke-Gogokhia ; KD Validated

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