Anticorps Polyclonal de lapin anti-DNM2

DNM2 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 14605-1-AP

Synonymes

CMTDI1, CMTDIB, DI CMTB, DNM2, DYN2, dynamin 2, DYNII

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Galerie de données de validation

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  WB analysis using 14605-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14605-1-AP (DNM2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 14605-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14605-1-AP (DNM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14605-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14605-1-AP (DNM2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14605-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14605-1-AP (DNM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14605-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14605-1-AP (DNM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules HeLa, cellules Jurkat, cellules MDA-MB-231, cellules NIH/3T3, plaquettes de sang périphérique humain, tissu cérébral de souris, tissu cérébral humain

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

14605-1-AP cible DNM2 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DNM2 Protéine recombinante Ag6156
• Nom complet: dynamin 2
• Masse moléculaire calculée: 98 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC054501
• Symbole du gène: DNM2
• Identification du gène (NCBI): 1785
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DNM2, also known as DYN2, DYNII, CMTDI1, CMTDIB, belongs to the dynamin family. DNM2 is a ubiquitously expressed large GTPase involved in clathrin-dependent and -independent endocytosis and intracellular membrane trafficking. DNM2 plays a role in the regulation of neuron morphology, axon growth, and the formation of neuronal growth cones. Dynamins are associated with microtubules. DNM2 plays an important role in endocytosis and is involved in cytokinesis (PMID: 33713620, 12498685). Mutations in DNM2 cause dominant centronuclear myopathy (PMID: 16227997). Defects in DNM2 are the cause of Charcot-Marie-Tooth disease dominant intermediate type B (CMTDIB) (PMID: 15731758). Alternate splicing results in multiple transcript variants.

Publications

Species	Application	Title
human	WB	Br J Pharmacol; CRP promotes atherosclerosis by increasing LDL transcytosis across endothelial cells.; Authors - Fang Bian
human	WB	J Transl Med; Angiopoietin2-mediated caveolin1 phosphorylation regulating transcytosis of renal tubular epithelial cell contributes to the occurrence of albuminuria under high glucose exposure.; Authors - Jing Liu
mouse	IF	Aging Cell; Degradation of cochlear Connexin26 accelerate the development of age-related hearing loss; Authors - Kai Xu