Anticorps Monoclonal anti-DNM2

DNM2 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1B8H3

N° de cat : 68209-1-PBS

Synonymes

dynamin2, Dynamin II, dynamin 2, DYN2, DNM 2

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Galerie de données de validation

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  WB analysis using 68209-1-Ig (same clone as 68209-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68209-1-Ig (DNM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68209-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68209-1-Ig (same clone as 68209-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DNM2 antibody (68209-1-Ig, Clone: 1B8H3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68209-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50954-1

  Cytometric bead array standard curve of MP50954-1, DNM2 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68209-1-PBS. Detection antibody: 68209-2-PBS. Standard:Ag6274. Range: 0.781-100 ng/mL.

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Informations sur le produit

68209-1-PBS cible DNM2 dans les applications de WB, IF/ICC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: DNM2 Protéine recombinante Ag6274
• Nom complet: dynamin 2
• Masse moléculaire calculée: 98 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC054501
• Symbole du gène: DNM2
• Identification du gène (NCBI): 1785
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DNM2, also known as DYN2, DYNII, CMTDI1, CMTDIB, belongs to the dynamin family. DNM2 is a ubiquitously expressed large GTPase involved in clathrin-dependent and -independent endocytosis and intracellular membrane trafficking. DNM2 plays a role in the regulation of neuron morphology, axon growth, and the formation of neuronal growth cones. Dynamins are associated with microtubules. DNM2 plays an important role in endocytosis and is involved in cytokinesis (PMID: 33713620, 12498685). Mutations in DNM2 cause dominant centronuclear myopathy (PMID: 16227997). Defects in DNM2 are the cause of Charcot-Marie-Tooth disease dominant intermediate type B (CMTDIB) (PMID: 15731758). Alternate splicing results in multiple transcript variants.

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