Anticorps Polyclonal de lapin anti-DNM3

• Phare
• Validé par KD/KO

DNM3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 14737-1-AP

Synonymes

DNM3, dynamin 3, Dynamin, testicular, KIAA0820, T dynamin

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Galerie de données de validation

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  WB analysis using 14737-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14737-1-AP (DNM3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 14737-1-AP

  IP result of anti-DNM3(IP:14737-1-AP, 4ug; Detection:14737-1-AP 1:5000) with mouse brain tissue lysate 1280 ug.

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  IP experiment of mouse brain using 14737-1-AP

  IP Result of anti-DNM3 (IP:14737-1-AP, 4ug; Detection:14737-1-AP 1:500) with mouse brain tissue lysate 2640ug.

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  IHC staining of human gliomas using 14737-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 14737-1-AP (DNM3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 14737-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 14737-1-AP (DNM3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse brain using 14737-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14737-1-AP (DNM3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 14737-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14737-1-AP (DNM3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 14737-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14737-1-AP (DNM3 antibody) at dilution of 1:50 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 14737-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14737-1-AP (DNM3 antibody) at dilution of 1:50 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 14737-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using DNM3 antibody (14737-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu cérébral de souris, tissu de cancer de l'estomac humain, tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 6 publications below

Informations sur le produit

14737-1-AP cible DNM3 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DNM3 Protéine recombinante Ag6381
• Nom complet: dynamin 3
• Masse moléculaire calculée: 98 kDa
• Poids moléculaire observé: 97-100 kDa
• Numéro d’acquisition GenBank: BC064546
• Symbole du gène: DNM3
• Identification du gène (NCBI): 26052
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

DNM3 (dynamin 3) is a member of dynamin proteins that participate in membrane rearrangements such as vesicular trafficking and phaocytosis. Three dynamin proteins encoded by different genes are present in mammals: DNM1 is mainly expressed in central nervous system, DNM2 ubiquitously expressed, and DNM3 expressed chiefly in the testis and brain. Western blot using this antibody specifically detected the 100 kDa DNM3 in mouse brain. It doesn't cross-react with other DNMs. (22977254, 21671749)

Publications

Species	Application	Title
	IF	Mol Cell Proteomics; Modification of Crocodile Spermatozoa Refutes the Tenet That Post-testicular Sperm Maturation Is Restricted To Mammals; Authors - Brett Nixon
mouse	IF	Mol Cell Proteomics; Proteomic profiling of crocodile spermatozoa refutes the tenet that post-testicular maturation is restricted to mammals.; Authors - Brett Nixon
mouse	WB,IHC,IF	J Biol Chem; Dynamin regulates specific membrane fusion events necessary for acrosomal exocytosis in mouse spermatozoa.; Authors - Reid Andrew T AT
human	IF	Mol Hum Reprod; Characterization of a novel role for the dynamin mechanoenzymes in the regulation of human sperm acrosomal exocytosis.; Authors - Wei Zhou
human	WB	Clin Exp Metastasis; Identification and validation of genes with expression patterns inverse to multiple metastasis suppressor genes in breast cancer cell lines.; Authors - Natascia Marino ; KD Validated
mouse	WB,IF	Biol Reprod; Developmental expression of the dynamin family of mechanoenzymes in the mouse epididymis.; Authors - Wei Zhou